DNA Origami Vaccine (DoriVac) Nanoparticles Improve Both Humoral and Cellular Immune Responses to Infectious Diseases
- Authors
- Zeng, Yang Claire; Young, Olivia; Xiong, Qiancheng; Si, Longlong; Ku, Min Wen; Bernier, Sylvie; Dembele, Hawa; Isinelli, Giorgia; Gilboa, Tal; Swank, Zoe; Seok, Su Hyun; Rajwar, Anjali; Jiang, Amanda; Zhai, Yunhao; Hellman, Caleb; Wintersinger, Chris; Graveline, Amanda; Williams, LaTonya; Vernet, Andyna; Sanchez, Melinda; Bardales, Sarai; Tomaras, Georgia; Ryu, Ju Hee; Kwon, Ick Chan; Goyal, Girija; Ingber, Donald; Shih, William
- Issue Date
- 2025-05
- Publisher
- Nature Publishing Group
- Citation
- Molecular Therapy, v.33, no.5
- Abstract
- Current SARS-CoV-2 vaccines have demonstrated robust induction of neutralizing antibodies and CD4+ T cell activation, however CD8+ responses are variable, and the duration of immunity and protection against variants are limited. Here we repurposed our DNA origami vaccine platform, DoriVac, for targeting infectious viruses, namely SARS-CoV-2, HIV, and Ebola. The DNA origami nanoparticle, conjugated with infectious-disease-specific HR2 peptides, which act as highly conserved antigens, and CpG adjuvant at precise nanoscale spacing, induced neutralizing antibodies, Th1 CD4+ T cells, and CD8+ T cells in na?ve mice, with significant improvement over a bolus control. Pre-clinical studies using lymph-node-on-a-chip systems validated that DoriVac, when conjugated with antigenic peptides or proteins, induced promising cellular immune responses in human cells. These results suggest that DoriVac holds potential as a versatile, modular vaccine platform, capable of inducing both humoral and cellular immunities. The programmability of this platform underscores its potential utility in addressing future pandemics.
- ISSN
- 1525-0016
- URI
- https://pubs.kist.re.kr/handle/201004/152713
- DOI
- 10.1101/2023.12.29.573647
- Appears in Collections:
- KIST Article > Others
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