Synthesis-enabled conformational assignment of natural N-acyl l-phenylalanine derivatives from freshwater sponge–associated Micromonospora sp. MS-62

Abstract

This study demonstrates a concise four-step total synthesis of N-acyl-l-phenylalanine derivatives (P1-P5) from Micromonospora sp. MS-62 enabling definitive assignment of the S configuration at C-1 ' via single-crystal X-ray diffraction. This synthesis clarifies the stereochemistry of this natural product class and facilitates future exploration of structure-activity relationship.