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dc.contributor.authorLee, Dahae-
dc.contributor.authorKim, Ji-Young-
dc.contributor.authorKwon, Hak Cheol-
dc.contributor.authorKwon, Jaeyoung-
dc.contributor.authorJang, Dae Sik-
dc.contributor.authorKang, Ki Sung-
dc.date.accessioned2024-01-12T03:32:08Z-
dc.date.available2024-01-12T03:32:08Z-
dc.date.created2022-03-29-
dc.date.issued2022-03-
dc.identifier.issn2223-7747-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/76777-
dc.description.abstractHerein, we determined whether alpha-Spinasterol, a stigmastane-type phytosterol isolated from Aster pseudoglehnii, potentially impacts glucose uptake and glucose-stimulated insulin secretion in skeletal muscle cells and pancreatic beta-cells, respectively. We observed that A. pseudoglehnii and its fractions enhanced glucose uptake, with no toxic effects on C2C12 cells, with the n-hexane fraction exhibiting the most potent effect. alpha-Spinasterol, isolated from the n-hexane fraction, enhanced glucose uptake with no toxic effects on C2C12 cells. Additionally, alpha-Spinasterol increased the expression of associated proteins, including insulin receptor substrate-1, AMP-activated protein kinase, and glucose transporter type 4, as determined by Western blotting. Furthermore, alpha-Spinasterol enhanced insulin secretion in response to high glucose concentrations, with no toxic effects on INS-1 cells; this effect was superior to that demonstrated by gliclazide (positive control), commonly prescribed to treat type 2 diabetes (T2D). alpha-Spinasterol enhanced the expression of associated proteins, including insulin receptor substrate-2, peroxisome proliferator-activated receptor gamma, and pancreatic and duodenal homeobox 1, as determined using Western blotting. The insulin secretory effect of alpha-Spinasterol was enhanced by a K+ channel blocker and L-type Ca2+ channel agonist and was suppressed by a K+ channel activator and L-type Ca2+ channel blocker. alpha-Spinasterol isolated from A. pseudoglehnii may improve hyperglycemia by improving glucose uptake into skeletal muscle cells and enhancing insulin secretion in pancreatic beta-cells. Accordingly, alpha-Spinasterol could be a potential candidate for anti-T2D therapy.-
dc.languageEnglish-
dc.publisherMDPI AG-
dc.titleDual Beneficial Effects of α-Spinasterol Isolated from Aster pseudoglehnii on Glucose Uptake in Skeletal Muscle Cells and Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells-
dc.typeArticle-
dc.identifier.doi10.3390/plants11050658-
dc.description.journalClass1-
dc.identifier.bibliographicCitationPlants, v.11, no.5-
dc.citation.titlePlants-
dc.citation.volume11-
dc.citation.number5-
dc.description.isOpenAccessY-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.identifier.wosid000772925000001-
dc.relation.journalWebOfScienceCategoryPlant Sciences-
dc.relation.journalResearchAreaPlant Sciences-
dc.type.docTypeArticle-
dc.subject.keywordPlusPPAR-GAMMA-
dc.subject.keywordPlusHYPERGLYCEMIA-
dc.subject.keywordPlusCOMBINATION-
dc.subject.keywordPlusACTIVATION-
dc.subject.keywordPlusGINSENG-
dc.subject.keywordAuthorAster pseudoglehnii-
dc.subject.keywordAuthoralpha-Spinasterol-
dc.subject.keywordAuthorglucose-stimulated insulin secretion-
dc.subject.keywordAuthorglucose uptake-
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