Proteomic analysis of human lung fibroblast cells exposed to diesel particulate matter using Tribrid mass spectrometer

Abstract

Proteomic analysis of human lung fibroblast cells exposed to diesel particulate matter using Tribrid mass spectrometer Jong Hwan Shin1, Yoon Jin Cho2,3, Mi Jung Ji1, Ji Hyun Back2,4, Yae Eun Park2, Hyuk Jeong3, Ji Eun Lee2*, and Hyun-Mee Park 1* 1Advanced Analysis Center, Korea Institute of Science and Technology 2Center for Theragnosis, Korea Institute of Science and Technology 3Department of chemistry, Sookmyung Women's University 4Department of Biotechnology, Korea University *Co-corresponding authors Particulate matter (PM) is known as carcinogen to humans. The International Agency for Research on Cancer (IARC) classified PM as potential toxicants, which are carcinogenic to humans. It is known that small-sized PM enters the human body through the both respiratory tract and causes disease. In order to explore the differentially expressed proteins associated with exposure of the PM, we pursued proteomic analysis of human lung fibroblast cells exposed to Diesel Particulate Matter (DPM) obtained from National Institute of Standards and Technology using Tribrid Orbitrap Eclipse mass spectrometry consisting of functioning mass analyzers, Quadrupole, ion trap and, orbitrap technology. Each mass analyzer must be able to work sequentially with the others which capable of performing CID, HCD or optional ETD fragmentations at any MSn stage with fragment ion detection in either ion trap or orbitrap. In the current study, human lung fibroblast cells (CCD-8Lu) were first exposed to different concentrations of DPM solutions (0, 100, 200, and 400 μg/mL in cell culture media) for 24 hrs and 48 hrs, respectively.