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dc.contributor.authorJung, Huijin-
dc.contributor.authorPark, Sung Hyeon-
dc.contributor.authorLee, Jiho-
dc.contributor.authorLee, Byeongdu-
dc.contributor.authorPark, Jinyoung-
dc.contributor.authorSeok, Youngung-
dc.contributor.authorChoi, Jong-Ho-
dc.contributor.authorKim, Min-Gon-
dc.contributor.authorSong, Chang-Seon-
dc.contributor.authorLee, Joonseok-
dc.date.accessioned2024-01-19T15:32:54Z-
dc.date.available2024-01-19T15:32:54Z-
dc.date.created2021-09-02-
dc.date.issued2021-01-
dc.identifier.issn0003-2700-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/117514-
dc.description.abstractIn this study, a signal-amplifiable nanoprobe-based chemiluminescent lateral flow immunoassay (CL-LFA) was developed to detect avian influenza viruses (AIV) and other contagious and fatal viral avian-origin diseases worldwide. Signal-amplifiable nanoprobes are capable of size-selective immobilization of antibodies (binding receptors) and enzymes (signal transducers) on sensitive paper-based sensor platforms. Particle structure designs and conjugation pathways conducive for antigen accessibility to maximum amounts of immobilized enzymes and antibodies have advanced. The detection limit of the CL-LFA using the signal-amplifiable nanoprobe for the nucleoprotein of the H3N2 virus was 5 pM. Sensitivity tests for low pathogenicity avian influenza H9N2, H1N1, and high pathogenicity avian influenza H5N9 viruses were conducted, and the detection limits of CL-LFA were found to be 10(3.5) 50% egg infective dose (EID50)/mL, 10(2.5) EID50/mL, and 10(4) EID50/mL, respectively, which is 20 to 100 times lower than that of a commercial AIV rapid test kit. Moreover, CL-LFA demonstrated high sensitivity and specificity against 37 clinical samples. The signal-amplifiable probe designed in this study is a potential diagnostic probe with ultrahigh sensitivity for applications in the field of clinical diagnosis, which requires sensitive antigen detection as evidenced by enhanced signaling capacity and sensitivity of the LFAs.-
dc.languageEnglish-
dc.publisherAMER CHEMICAL SOC-
dc.titleA Size-Selectively Biomolecule-Immobilized Nanoprobe-Based Chemiluminescent Lateral Flow Immunoassay for Detection of Avian-Origin Viruses-
dc.typeArticle-
dc.identifier.doi10.1021/acs.analchem.0c03153-
dc.description.journalClass1-
dc.identifier.bibliographicCitationANALYTICAL CHEMISTRY, v.93, no.2, pp.792 - 800-
dc.citation.titleANALYTICAL CHEMISTRY-
dc.citation.volume93-
dc.citation.number2-
dc.citation.startPage792-
dc.citation.endPage800-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.identifier.wosid000612336200020-
dc.identifier.scopusid2-s2.0-85096577008-
dc.relation.journalWebOfScienceCategoryChemistry, Analytical-
dc.relation.journalResearchAreaChemistry-
dc.type.docTypeArticle-
dc.subject.keywordPlusNANOPARTICLES-
dc.subject.keywordPlusDELIVERY-
dc.subject.keywordPlusSYSTEM-
dc.subject.keywordPlusRESONANCE ENERGY-TRANSFER-
dc.subject.keywordPlusINFLUENZA-VIRUS-
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KIST Article > 2021
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