Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Jung, Huijin | - |
dc.contributor.author | Park, Sung Hyeon | - |
dc.contributor.author | Lee, Jiho | - |
dc.contributor.author | Lee, Byeongdu | - |
dc.contributor.author | Park, Jinyoung | - |
dc.contributor.author | Seok, Youngung | - |
dc.contributor.author | Choi, Jong-Ho | - |
dc.contributor.author | Kim, Min-Gon | - |
dc.contributor.author | Song, Chang-Seon | - |
dc.contributor.author | Lee, Joonseok | - |
dc.date.accessioned | 2024-01-19T15:32:54Z | - |
dc.date.available | 2024-01-19T15:32:54Z | - |
dc.date.created | 2021-09-02 | - |
dc.date.issued | 2021-01 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | https://pubs.kist.re.kr/handle/201004/117514 | - |
dc.description.abstract | In this study, a signal-amplifiable nanoprobe-based chemiluminescent lateral flow immunoassay (CL-LFA) was developed to detect avian influenza viruses (AIV) and other contagious and fatal viral avian-origin diseases worldwide. Signal-amplifiable nanoprobes are capable of size-selective immobilization of antibodies (binding receptors) and enzymes (signal transducers) on sensitive paper-based sensor platforms. Particle structure designs and conjugation pathways conducive for antigen accessibility to maximum amounts of immobilized enzymes and antibodies have advanced. The detection limit of the CL-LFA using the signal-amplifiable nanoprobe for the nucleoprotein of the H3N2 virus was 5 pM. Sensitivity tests for low pathogenicity avian influenza H9N2, H1N1, and high pathogenicity avian influenza H5N9 viruses were conducted, and the detection limits of CL-LFA were found to be 10(3.5) 50% egg infective dose (EID50)/mL, 10(2.5) EID50/mL, and 10(4) EID50/mL, respectively, which is 20 to 100 times lower than that of a commercial AIV rapid test kit. Moreover, CL-LFA demonstrated high sensitivity and specificity against 37 clinical samples. The signal-amplifiable probe designed in this study is a potential diagnostic probe with ultrahigh sensitivity for applications in the field of clinical diagnosis, which requires sensitive antigen detection as evidenced by enhanced signaling capacity and sensitivity of the LFAs. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | A Size-Selectively Biomolecule-Immobilized Nanoprobe-Based Chemiluminescent Lateral Flow Immunoassay for Detection of Avian-Origin Viruses | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/acs.analchem.0c03153 | - |
dc.description.journalClass | 1 | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, v.93, no.2, pp.792 - 800 | - |
dc.citation.title | ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 93 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 792 | - |
dc.citation.endPage | 800 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.identifier.wosid | 000612336200020 | - |
dc.identifier.scopusid | 2-s2.0-85096577008 | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | RESONANCE ENERGY-TRANSFER | - |
dc.subject.keywordPlus | INFLUENZA-VIRUS | - |
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