A Size-Selectively Biomolecule-Immobilized Nanoprobe-Based Chemiluminescent Lateral Flow Immunoassay for Detection of Avian-Origin Viruses
- Authors
- Jung, Huijin; Park, Sung Hyeon; Lee, Jiho; Lee, Byeongdu; Park, Jinyoung; Seok, Youngung; Choi, Jong-Ho; Kim, Min-Gon; Song, Chang-Seon; Lee, Joonseok
- Issue Date
- 2021-01
- Publisher
- AMER CHEMICAL SOC
- Citation
- ANALYTICAL CHEMISTRY, v.93, no.2, pp.792 - 800
- Abstract
- In this study, a signal-amplifiable nanoprobe-based chemiluminescent lateral flow immunoassay (CL-LFA) was developed to detect avian influenza viruses (AIV) and other contagious and fatal viral avian-origin diseases worldwide. Signal-amplifiable nanoprobes are capable of size-selective immobilization of antibodies (binding receptors) and enzymes (signal transducers) on sensitive paper-based sensor platforms. Particle structure designs and conjugation pathways conducive for antigen accessibility to maximum amounts of immobilized enzymes and antibodies have advanced. The detection limit of the CL-LFA using the signal-amplifiable nanoprobe for the nucleoprotein of the H3N2 virus was 5 pM. Sensitivity tests for low pathogenicity avian influenza H9N2, H1N1, and high pathogenicity avian influenza H5N9 viruses were conducted, and the detection limits of CL-LFA were found to be 10(3.5) 50% egg infective dose (EID50)/mL, 10(2.5) EID50/mL, and 10(4) EID50/mL, respectively, which is 20 to 100 times lower than that of a commercial AIV rapid test kit. Moreover, CL-LFA demonstrated high sensitivity and specificity against 37 clinical samples. The signal-amplifiable probe designed in this study is a potential diagnostic probe with ultrahigh sensitivity for applications in the field of clinical diagnosis, which requires sensitive antigen detection as evidenced by enhanced signaling capacity and sensitivity of the LFAs.
- Keywords
- NANOPARTICLES; DELIVERY; SYSTEM; RESONANCE ENERGY-TRANSFER; INFLUENZA-VIRUS
- ISSN
- 0003-2700
- URI
- https://pubs.kist.re.kr/handle/201004/117514
- DOI
- 10.1021/acs.analchem.0c03153
- Appears in Collections:
- KIST Article > 2021
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