Dependency of Experimental Autoimmune Encephalomyelitis Induction on MOG(35-55) Properties Modulating Matrix Metalloproteinase-9 and Interleukin-6

Abstract

Experimental autoimmune encephalomyelitis (EAE) is commonly induced with myelin oligodendrocyte glycoprotein (MOG)(35-55); occasionally, EAE is not well induced despite MOG(35-55) immunization. To confirm that EAE induction varies with difference in MOG(35-55) properties, we compared three MOG(35-55) from different commercial sources, which are MOG-A, MOG-B, and MOG-C. The peptides induced EAE disease with 100, 40, and 20 % incidence, respectively. Compared with others, MOG-A showed higher peptide purity (99.2 %) and content (92.2 %) and presented a sheet shape with additional sodium and chloride chemical elements. In MOG-A-treated group, MMP-9 activity and IL-6 levels were considerably higher than the other groups in CNS tissues, and significantly increased VCAM-1, IFN-gamma, and decreased IL-4 were also shown compared to MOG-B- and/or MOG-C-treated group. In conclusion, the immunological and toxicological changes by the difference in MOG(35-55) properties modulate EAE induction, and MOG(35-55) which affects MMP-9 activity and IL-6 levels may be the most effective EAE-inducing antigen. This study can be potentially applied by researchers using MOG(35-55) peptide and manufacturers for MOG(35-55) synthesis.