Effect of temperature on continuous fermentative hydrogen production from Laminaria japonica by anaerobic mixed cultures

Authors
Shi, XueqingKim, Dong-HoonShin, Hang-SikJung, Kyung-Won
Issue Date
2013-09
Publisher
ELSEVIER SCI LTD
Citation
BIORESOURCE TECHNOLOGY, v.144, pp.225 - 231
Abstract
The temperature effect on continuous dark fermentative hydrogen production from non-pretreated Laminaria japonica was investigated in the present study. In a preliminary step, the fermentors were continuously operated as an inoculation process at three different temperatures, 35, 50 and 65 degrees C, to respectively represent mesophilic, thermophilic, and hyperthermophilic conditions. An optimization process was subsequently conducted with a range of organic loading rate (OLR) and cultivation pH. Among the various operation conditions, the maximum H-2 yield, 61.3 +/- 2.0 mL H-2/g TS, was observed under a mesophilic condition at OLR of 3.4 g COD/L/d and pH 5.5. From a PCR-DGGE analysis, it was found that an increase of temperature can reduce the microbial diversity and change the predominant species. Finally, total cellulase activity was measured, to investigate the effect of temperature on hydrolysis of L. japonica. The highest cellulase activity was 0.19 +/- 0.02 FPU/mL, observed at 35 degrees C, coinciding with the maximum H-2 yield. (c) 2013 Elsevier Ltd. All rights reserved.
Keywords
TRICHODERMA-REESEI; LIGNOCELLULOSIC BIOMASS; BIOHYDROGEN PRODUCTION; CORNSTALK WASTES; PRETREATMENT; CELLULASES; CONVERSION; TRICHODERMA-REESEI; LIGNOCELLULOSIC BIOMASS; BIOHYDROGEN PRODUCTION; CORNSTALK WASTES; PRETREATMENT; CELLULASES; CONVERSION; Dark fermentative hydrogen production; Laminaria japonica; Mixed cultures; Temperature; Total cellulase activity
ISSN
0960-8524
URI
https://pubs.kist.re.kr/handle/201004/127723
DOI
10.1016/j.biortech.2013.06.107
Appears in Collections:
KIST Article > 2013
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