Triglyceride down-regulates matrix metalloprotease-12 expression in THP-1 macrophages through activation of the NF-kappa B pathway

Abstract

Triglyceride (TG) is known to be associated with inflammatory diseases including atherosclerosis. At the cellular level, TG can act as an immunomodulatory stimulus for macrophages. In this study we show that TG treatment of PMA-differentiated macrophages resulted in down-regulation of MMP-12 expression in a time- and dose-dependent manner. MMP-19 expression was unaffected by TG treatment. Using a variety of chemical inhibitors for cell signaling pathways we demonstrate that TG-induced down-regulation of MMP-12 occurs in part by activation of the NF-kappa B pathway. Finally, TG treatment of PMA-differentiated macrophages decreased cell migration in a wound healing assay. Taken together our data suggests that one function of TG is to modulate macrophage migration in tissues.