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dc.contributor.authorLee, Hye-Jin-
dc.contributor.authorKim, Byoung Chan-
dc.contributor.authorOh, Min-Kyu-
dc.contributor.authorKim, Jungbae-
dc.date.accessioned2024-01-20T14:34:14Z-
dc.date.available2024-01-20T14:34:14Z-
dc.date.created2021-09-04-
dc.date.issued2012-06-
dc.identifier.issn1359-7345-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/129219-
dc.description.abstractStable enzyme precipitate coatings (EPCs) with high enzyme loading were employed to develop a sensitive and reliable detection protocol, termed EPC-linked aptamer assay (EPC-LAA). EPC-LAA achieved the limit of detection to be 0.5 ng per mL of thrombin (13.5 pM), and maintained a reasonably stable detection signal even after storing all the reagents at 40 degrees C for 66 days.-
dc.languageEnglish-
dc.publisherROYAL SOC CHEMISTRY-
dc.subjectPROTEIN-DETECTION-
dc.subjectNEGATIVE COOPERATIVITY-
dc.titleA sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay-
dc.typeArticle-
dc.identifier.doi10.1039/c2cc30710c-
dc.description.journalClass1-
dc.identifier.bibliographicCitationCHEMICAL COMMUNICATIONS, v.48, no.48, pp.5971 - 5973-
dc.citation.titleCHEMICAL COMMUNICATIONS-
dc.citation.volume48-
dc.citation.number48-
dc.citation.startPage5971-
dc.citation.endPage5973-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.identifier.wosid000304263600003-
dc.identifier.scopusid2-s2.0-84861632731-
dc.relation.journalWebOfScienceCategoryChemistry, Multidisciplinary-
dc.relation.journalResearchAreaChemistry-
dc.type.docTypeArticle-
dc.subject.keywordPlusPROTEIN-DETECTION-
dc.subject.keywordPlusNEGATIVE COOPERATIVITY-
dc.subject.keywordAuthorAptamer-
dc.subject.keywordAuthorThrombin-
dc.subject.keywordAuthorenzyme-
dc.subject.keywordAuthorprecipitate coating-
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