Novel Cyanine Dyes with Vinylsulfone Group for Labeling Biomolecules
- Authors
- Park, Jin Woo; Kim, YoungSoo; Lee, Kee-Jung; Kim, Dong Jin
- Issue Date
- 2012-03
- Publisher
- AMER CHEMICAL SOC
- Citation
- BIOCONJUGATE CHEMISTRY, v.23, no.3, pp.350 - 362
- Abstract
- Novel vinylsulfone cyanine dyes (em. 550-850 nm) were designed and synthesized for fluorescent labeling of biomolecules via 1,2-addition reaction in aqueous conditions. Due to the virtue of chemical structures of both fluorophore and reactive group, these dyes could be significantly stable and reactive in various aqueous/organic conditions. A wide variety of pH, temperature, buffer concentration, and protein were tested for the optimal labeling condition.
- Keywords
- N-HYDROXYSUCCINIMIDE ESTERS; REAGENTS; KINETICS; PROBES; DNA; N-HYDROXYSUCCINIMIDE ESTERS; REAGENTS; KINETICS; PROBES; DNA; 형광물질; 염료; fluorescence; vinylsulfone
- ISSN
- 1043-1802
- URI
- https://pubs.kist.re.kr/handle/201004/129480
- DOI
- 10.1021/bc200232d
- Appears in Collections:
- KIST Article > 2012
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.