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A novel real-time PCR method based on signaling-by-incorporation

Authors
Kim, Da-Rae; Ahn, Hee-Chul; Lee, Won-Ja; Ahn, Dae-Ro
Issue Date
2011-01
Publisher
ROYAL SOC CHEMISTRY
Citation
CHEMICAL COMMUNICATIONS, v.47, no.2, pp.791 - 793
Abstract
We describe a novel real-time PCR method, which utilizes the in situ generated signal upon incorporation of a fluorogenic nucleotide. The method has been compared with a conventional method and evaluated for practical uses such as quantification of cDNA and malaria diagnosis.
Keywords
SYBR-GREEN-I; MOLECULAR BEACONS; FLUORESCENCE; QUANTIFICATION; HYBRIDIZATION; SYBR-GREEN-I; MOLECULAR BEACONS; FLUORESCENCE; QUANTIFICATION; HYBRIDIZATION
ISSN
1359-7345
URI
https://pubs.kist.re.kr/handle/201004/130792
DOI
10.1039/c0cc04516k
Appears in Collections:
KIST Article > 2011
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