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dc.contributor.authorJung, Hyun-Jin-
dc.contributor.authorLee, Won-Yong-
dc.contributor.authorChung, Bong Chul-
dc.contributor.authorChoi, Man Ho-
dc.date.accessioned2024-01-20T22:01:04Z-
dc.date.available2024-01-20T22:01:04Z-
dc.date.created2021-09-03-
dc.date.issued2009-02-27-
dc.identifier.issn0021-9673-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/132727-
dc.description.abstractAn efficient analytical method for simultaneous determination of 12 SFEs in serum is described. The method involves solid-phase extraction to isolate of SFEs from interfering species, especially cholesteryl esters, conversion to trimethylsilyl (TMS) ether derivatives for the direct analysis by gas chromatography-mass spectrometry (GC-MS) using a high temperature MXT-1 (Silcosteel-treated stainless steel) capillary column. All SFEs as their TMS derivatives were well separated with excellent peak shapes within 12 min. Overall recoveries ranged from 88% to 119%, with a detection limits for SFEs ranged from 2 to 30 mu g L-1. The linearity as correlation coefficient was higher than 0.99 except for pregnenolone-3-arachidate (r(2) = -0.98) in the concentration range of 5-3000 mu g L-1. Ten serum samples obtained from volunteers were also analyzed and quantitatively determined of DHEA-3-palmitate and pregnenolone-3-stearate in 1.8-1195.8 mu g L-1 concentration. The devised high temperature GC-MS method could be useful for identification of SFEs in biological specimens including serum. (C) 2008 Elsevier B.V. All rights reserved.-
dc.languageEnglish-
dc.publisherELSEVIER SCIENCE BV-
dc.subjectLIQUID-CHROMATOGRAPHY-
dc.subjectSAMPLE PREPARATION-
dc.subjectFOLLICULAR-FLUID-
dc.subjectPREGNENOLONE-
dc.subjectIDENTIFICATION-
dc.subjectESTRADIOL-
dc.subjectRAT-
dc.subjectESTERIFICATION-
dc.subjectNEUROSTEROIDS-
dc.subjectLIPOPROTEINS-
dc.titleMass spectrometric profiling of saturated fatty acid esters of steroids separated by high-temperature gas chromatography-
dc.typeArticle-
dc.identifier.doi10.1016/j.chroma.2008.12.059-
dc.description.journalClass1-
dc.identifier.bibliographicCitationJOURNAL OF CHROMATOGRAPHY A, v.1216, no.9, pp.1463 - 1468-
dc.citation.titleJOURNAL OF CHROMATOGRAPHY A-
dc.citation.volume1216-
dc.citation.number9-
dc.citation.startPage1463-
dc.citation.endPage1468-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.identifier.wosid000263610500025-
dc.identifier.scopusid2-s2.0-59049106573-
dc.relation.journalWebOfScienceCategoryBiochemical Research Methods-
dc.relation.journalWebOfScienceCategoryChemistry, Analytical-
dc.relation.journalResearchAreaBiochemistry & Molecular Biology-
dc.relation.journalResearchAreaChemistry-
dc.type.docTypeArticle-
dc.subject.keywordPlusLIQUID-CHROMATOGRAPHY-
dc.subject.keywordPlusSAMPLE PREPARATION-
dc.subject.keywordPlusFOLLICULAR-FLUID-
dc.subject.keywordPlusPREGNENOLONE-
dc.subject.keywordPlusIDENTIFICATION-
dc.subject.keywordPlusESTRADIOL-
dc.subject.keywordPlusRAT-
dc.subject.keywordPlusESTERIFICATION-
dc.subject.keywordPlusNEUROSTEROIDS-
dc.subject.keywordPlusLIPOPROTEINS-
dc.subject.keywordAuthorSteroids-
dc.subject.keywordAuthorFatty acids-
dc.subject.keywordAuthorLipoidal conjugate-
dc.subject.keywordAuthorGas chromatography-
dc.subject.keywordAuthorMass spectrometry-
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