Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Jung, Hyun-Jin | - |
dc.contributor.author | Lee, Won-Yong | - |
dc.contributor.author | Chung, Bong Chul | - |
dc.contributor.author | Choi, Man Ho | - |
dc.date.accessioned | 2024-01-20T22:01:04Z | - |
dc.date.available | 2024-01-20T22:01:04Z | - |
dc.date.created | 2021-09-03 | - |
dc.date.issued | 2009-02-27 | - |
dc.identifier.issn | 0021-9673 | - |
dc.identifier.uri | https://pubs.kist.re.kr/handle/201004/132727 | - |
dc.description.abstract | An efficient analytical method for simultaneous determination of 12 SFEs in serum is described. The method involves solid-phase extraction to isolate of SFEs from interfering species, especially cholesteryl esters, conversion to trimethylsilyl (TMS) ether derivatives for the direct analysis by gas chromatography-mass spectrometry (GC-MS) using a high temperature MXT-1 (Silcosteel-treated stainless steel) capillary column. All SFEs as their TMS derivatives were well separated with excellent peak shapes within 12 min. Overall recoveries ranged from 88% to 119%, with a detection limits for SFEs ranged from 2 to 30 mu g L-1. The linearity as correlation coefficient was higher than 0.99 except for pregnenolone-3-arachidate (r(2) = -0.98) in the concentration range of 5-3000 mu g L-1. Ten serum samples obtained from volunteers were also analyzed and quantitatively determined of DHEA-3-palmitate and pregnenolone-3-stearate in 1.8-1195.8 mu g L-1 concentration. The devised high temperature GC-MS method could be useful for identification of SFEs in biological specimens including serum. (C) 2008 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | LIQUID-CHROMATOGRAPHY | - |
dc.subject | SAMPLE PREPARATION | - |
dc.subject | FOLLICULAR-FLUID | - |
dc.subject | PREGNENOLONE | - |
dc.subject | IDENTIFICATION | - |
dc.subject | ESTRADIOL | - |
dc.subject | RAT | - |
dc.subject | ESTERIFICATION | - |
dc.subject | NEUROSTEROIDS | - |
dc.subject | LIPOPROTEINS | - |
dc.title | Mass spectrometric profiling of saturated fatty acid esters of steroids separated by high-temperature gas chromatography | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.chroma.2008.12.059 | - |
dc.description.journalClass | 1 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CHROMATOGRAPHY A, v.1216, no.9, pp.1463 - 1468 | - |
dc.citation.title | JOURNAL OF CHROMATOGRAPHY A | - |
dc.citation.volume | 1216 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1463 | - |
dc.citation.endPage | 1468 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.identifier.wosid | 000263610500025 | - |
dc.identifier.scopusid | 2-s2.0-59049106573 | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | LIQUID-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | SAMPLE PREPARATION | - |
dc.subject.keywordPlus | FOLLICULAR-FLUID | - |
dc.subject.keywordPlus | PREGNENOLONE | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | ESTRADIOL | - |
dc.subject.keywordPlus | RAT | - |
dc.subject.keywordPlus | ESTERIFICATION | - |
dc.subject.keywordPlus | NEUROSTEROIDS | - |
dc.subject.keywordPlus | LIPOPROTEINS | - |
dc.subject.keywordAuthor | Steroids | - |
dc.subject.keywordAuthor | Fatty acids | - |
dc.subject.keywordAuthor | Lipoidal conjugate | - |
dc.subject.keywordAuthor | Gas chromatography | - |
dc.subject.keywordAuthor | Mass spectrometry | - |
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