Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Kim, Choong | - |
dc.contributor.author | Lee, In Hye | - |
dc.contributor.author | Lee, Kangsun | - |
dc.contributor.author | Ryu, Sung Shin | - |
dc.contributor.author | Lee, Sang Ho | - |
dc.contributor.author | Lee, Kyu-Jung | - |
dc.contributor.author | Lee, Jinwoo | - |
dc.contributor.author | Kang, Ji Yoon | - |
dc.contributor.author | Kim, Tae Song | - |
dc.date.accessioned | 2024-01-21T00:04:20Z | - |
dc.date.available | 2024-01-21T00:04:20Z | - |
dc.date.created | 2021-09-02 | - |
dc.date.issued | 2007-12 | - |
dc.identifier.issn | 0916-8451 | - |
dc.identifier.uri | https://pubs.kist.re.kr/handle/201004/133944 | - |
dc.description.abstract | A multi-well chip (MWC) is described by which mouse embryonic carcinoma (EC) stem cells form a comparatively more rapid and uniform embryoid body (EB) over the conventional hanging drop (HD) method. The newly developed MWC consists of an array of extruded through-holes, each of which holds a droplet of the cell suspension. The study found that the small curvature radius of the droplet in the MWC improved the EB formation rate of a hanging drop from 70% to 98%. Furthermore, the EBs formed by the MWC were uniformly round in shape regardless of the number of suspended cells ranging from 0.5 x 10(3) to 20 x 10(3). The ratio of beating colonies from the MWC was over 2-fold larger than that from HD. The experiments demonstrate that the MWC will be a valuable experimental tool for robust and reproducible EB-based differentiation of a defined number of ES cells. | - |
dc.language | English | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.subject | IN-VITRO | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | CARDIOMYOCYTES | - |
dc.subject | EFFICIENCY | - |
dc.subject | CULTURE | - |
dc.subject | BODIES | - |
dc.title | Multi-well chip for forming a uniform embryoid body in a tiny droplet with mouse embryonic stem cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1271/bbb.70384 | - |
dc.description.journalClass | 1 | - |
dc.identifier.bibliographicCitation | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.71, no.12, pp.2985 - 2991 | - |
dc.citation.title | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | - |
dc.citation.volume | 71 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 2985 | - |
dc.citation.endPage | 2991 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.identifier.wosid | 000252024400015 | - |
dc.identifier.scopusid | 2-s2.0-37649016589 | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | CARDIOMYOCYTES | - |
dc.subject.keywordPlus | EFFICIENCY | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | BODIES | - |
dc.subject.keywordAuthor | embryoid body | - |
dc.subject.keywordAuthor | multi-well chip | - |
dc.subject.keywordAuthor | hanging drop | - |
dc.subject.keywordAuthor | differentiation | - |
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