젤방사성형법에 의한 혈관용 스캐폴드의 제조 및 특성

Abstract

We developed a biodegradable porous scaffold fabricated by a gel spinning method using a biodegradable and elastic polymer, poly(L-lactide-co-ε-caprolactone) (PLCL) (50:50) for vascular grafts application. To characterize the scaffold, we tested fiber thickness, SEM, cell seeding efficiency, tensile properties, and elasticity. In seeding test, we used bone marrow mononuclear cells isolated from rabbit. Furthermore, we examined tissue ingrowth activities for the scaffolds implanted in nude mice. The fiber thickness of the gel-spun scaffolds increased as the concentration of polymer solution increased. In mechanical properties such as tensile strength and elasticity, the gel-spun scaffolds were superior to scaffolds fabricated by a extrusion technique. In addition, the cell seeding efficiency of the scaffolds was higher than that of other scaffolds fabricated by compared methods. In implantation experiments, the scaffolds showed a good tissue ingrowth through open spaces between micro fibers. In conclusion, gel-spun tubular scaffolds can be a good candidate as scaffolds for cardiovascular tissue engineering.