아임계/초임계 수에 의한 토종콩으로부터 이소플라본의 추출

Abstract

Aglycones compounds were extracted from Korean soybean by sub/supercritical water. The various experimental variables were the temperature and pressure of sub/supercritical water. The extracted sample was analyzed by reversed-phase high performance liquid chromatography (RP-HPLC). Aglycons were selectively extracted at the experimental condition of 200 oC and 200 bar of subcritical water. It was also experimentally observed that almost the same amount of aglycones, 0.9 µg, was extracted from 5 g of Korean soybean at 400 oC and 250 bar of supercritical water.