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dc.contributor.authorHan, SJ-
dc.contributor.authorBack, JH-
dc.contributor.authorYoon, MY-
dc.contributor.authorShin, PK-
dc.contributor.authorCheong, CS-
dc.contributor.authorSung, MH-
dc.contributor.authorHong, SP-
dc.contributor.authorChung, IY-
dc.contributor.authorHan, YS-
dc.date.accessioned2024-01-21T09:02:26Z-
dc.date.available2024-01-21T09:02:26Z-
dc.date.created2021-09-03-
dc.date.issued2003-05-
dc.identifier.issn0300-9084-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/138613-
dc.description.abstractA novel lipase gene, lipase A, of Acinetobacter species SY-01 (A. species SY-01) was cloned, sequenced, and expressed in Bacillus subtilis 168. The deduced amino acid (aa) sequences for the lipase A and its chaperone, lipase-specific chaperone, were found to encode mature proteins of 339 aa (37.2 kDa) and 347 aa (38.1 kDa), respectively. The aa sequence of lipase A and lipase-specific chaperone shared high homology 82 and 67% identity with the lipase A and the lipase B of A. species RAG-1. This new lipase was defined as a group I Proteobacterial lipase family. The expressed lipase A was purified through sequential treatment with Q-Sepharose, Resource Q, and Superdex-S75 columns. The maximal activity was observed at 50 degreesC for hydrolysis of p-nitrophenyl monoesters and found to be stable at pH 9-11, with optimal activity at pH 10. Lipase A hydrolyzed wide range of fatty acid esters of p-nitrophenyl, but preferentially hydrolyzed short length acyl chains (C2 and C4). Moreover, lipase A from A. species SY-01 catalyzed hydrolysis of the two acetate isomers of cis-(+/-)-2-(bromomethyl)-2-(2,4-dichloro phenyl)-1,3-dioxolane-4-methyl acetate, an intermediate required for the synthesis of Itraconazole which was an anti-fungal drug, at different rate and yielded cis-(-)-isomer in 81.5% conversion with 91.9% enantiomeric excess. (C) 2003 Editions scientifiques et medicales Elsevier SAS and Societe francaise de biochimie et biologic moleculaire. All rights reserved.-
dc.languageEnglish-
dc.publisherEDITIONS SCIENTIFIQUES MEDICALES ELSEVIER-
dc.subjectPSEUDOMONAS-GLUMAE LIPASE-
dc.subjectEXTRACELLULAR LIPASE-
dc.subjectMOLECULAR-CLONING-
dc.subjectBACTERIAL LIPASES-
dc.subjectIN-VITRO-
dc.subjectSEQUENCE-ANALYSIS-
dc.subjectESCHERICHIA-COLI-
dc.subjectGENE-EXPRESSION-
dc.subjectPURIFICATION-
dc.subjectAERUGINOSA-
dc.titleExpression and characterization of a novel enantioselective lipase from Acinetobacter species SY-01-
dc.typeArticle-
dc.identifier.doi10.1016/S0300-9084(03)00057-9-
dc.description.journalClass1-
dc.identifier.bibliographicCitationBIOCHIMIE, v.85, no.5, pp.501 - 510-
dc.citation.titleBIOCHIMIE-
dc.citation.volume85-
dc.citation.number5-
dc.citation.startPage501-
dc.citation.endPage510-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.identifier.wosid000183355900003-
dc.identifier.scopusid2-s2.0-0037809707-
dc.relation.journalWebOfScienceCategoryBiochemistry & Molecular Biology-
dc.relation.journalResearchAreaBiochemistry & Molecular Biology-
dc.type.docTypeArticle-
dc.subject.keywordPlusPSEUDOMONAS-GLUMAE LIPASE-
dc.subject.keywordPlusEXTRACELLULAR LIPASE-
dc.subject.keywordPlusMOLECULAR-CLONING-
dc.subject.keywordPlusBACTERIAL LIPASES-
dc.subject.keywordPlusIN-VITRO-
dc.subject.keywordPlusSEQUENCE-ANALYSIS-
dc.subject.keywordPlusESCHERICHIA-COLI-
dc.subject.keywordPlusGENE-EXPRESSION-
dc.subject.keywordPlusPURIFICATION-
dc.subject.keywordPlusAERUGINOSA-
dc.subject.keywordAuthorAcinetobacter species SY-01-
dc.subject.keywordAuthorlipase-
dc.subject.keywordAuthorlipase-specific chaperone-
dc.subject.keywordAuthorenantioselective-
dc.subject.keywordAuthorcis-(+/-)-2-(bromomethyl)-2-(2,4-dichlorophenyl)-1,3-dioxolane4-methyl acetate-
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