CHROMATOGRAPHIC BEHAVIOR OF DEOXYRIBONUCLEOSIDES WITH RESPECT TO ORGANIC MODIFIER CONTENT IN THE MOBILE-PHASE

Abstract

A chromatographic behaviour of five deoxyribonucleosides (dCyd, dUrd, dGuo, dThd, and dAdo) has been studied under isocratic conditions of reversed-phase HPLC. The Waters column packed by 10 mu m mu-Bondapak C18 was used. The volume fraction (F) of organic modifier was changed from 0.05 to 0.30, and to 0.12 for methanol and acetonitrile, respectively. Furthermore, the capacity factors with a mobile phase of 100 % water (k'(w)) have been measured. Linear regression has been carried out for the log k' vs. F data and the intercepts and the slopes of these plots were calculated. The slope magnitudes of different solutes were almost the same for each organic modifier. In all cases, the experimental values of k'(w) were higher than extrapolated ones. For each solute, extrapolation of log k' vs. F gives practically the same intercept values for both organic modifiers. It was concluded that the intercepts calculated in the region of small content of organic modifier are independent on the nature of organic modifier and correlate with deoxyribonucleosides nature only.