Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | RHIM, H | - |
dc.contributor.author | MILLER, RJ | - |
dc.date.accessioned | 2024-01-21T21:16:24Z | - |
dc.date.available | 2024-01-21T21:16:24Z | - |
dc.date.created | 2021-09-01 | - |
dc.date.issued | 1994-12 | - |
dc.identifier.issn | 0270-6474 | - |
dc.identifier.uri | https://pubs.kist.re.kr/handle/201004/145453 | - |
dc.description.abstract | We have investigated the coupling between opioid receptors and different types of Ca2+ channels in neurons acutely isolated from the nucleus tractus solitarius (NTS) of the rat. Using fura-2-based imaging we found that Ca2+ transients evoked by depolarization with 50 mM KCl were suppressed by the mu-opioid receptor agonist D-Ala(2),N-MePhe(4),Gly(5)-ol-enkephalin (DAMGO) and less effectively by the kappa-receptor agonist U-69,593. The delta-receptor agonist D-Pen(2),D-Pen(5)-enkephalin (DPDPE) was ineffective. In whole-cell voltage-clamp recordings from these neurons, depolarizing voltage steps elicited high-threshold Ca2+ currents that could be distinguished pharmacologically into different components. Part of the current could be blocked by dihydropyridines, part by omega-conotoxin-GVIA and part by omega-agatoxin-IVA. This suggests that the neurons contained L-, N-, and P/Q-type Ca2+ channels. DAMGO and U-69,593 both blocked part of the Ca2+ current but DPDPE was ineffective. Perfusion of GTP-gamma-S into the cells produced a rapid rundown of the Ca2+ current and occluded further effects of the opioid agonists, suggesting the involvement of a G-protein in the coupling mechanism. Inhibition of L-channels did not alter the effect of DAMGO. On the other hand inhibition of N-channels occluded about 80% of the effect of DAMGO. Inhibition of the P/Q-current occluded the remainder of the DAMGO effect. Thus, it appears that activation of opioid receptors can inhibit N- and P/Q-type Ca2+ channels but not L-channels in these cells. It is likely that such effects are important in opioid-mediated inhibition of transmitter release in the brain. | - |
dc.language | English | - |
dc.publisher | OXFORD UNIV PRESS INC | - |
dc.subject | METABOTROPIC GLUTAMATE-RECEPTOR | - |
dc.subject | SYNAPTIC TRANSMISSION | - |
dc.subject | CA2+ CHANNELS | - |
dc.subject | N-TYPE | - |
dc.subject | PRESYNAPTIC INHIBITION | - |
dc.subject | FUNCTIONAL EXPRESSION | - |
dc.subject | PHARMACOLOGICAL PROPERTIES | - |
dc.subject | NEUROTRANSMITTER RELEASE | - |
dc.subject | HIPPOCAMPAL-NEURONS | - |
dc.subject | MEDIATED INHIBITION | - |
dc.title | OPIOID RECEPTORS MODULATE DIVERSE TYPES OF CALCIUM CHANNELS IN THE NUCLEUS-TRACTUS-SOLITARIUS OF THE RAT | - |
dc.type | Article | - |
dc.description.journalClass | 1 | - |
dc.identifier.bibliographicCitation | JOURNAL OF NEUROSCIENCE, v.14, no.12, pp.7608 - 7615 | - |
dc.citation.title | JOURNAL OF NEUROSCIENCE | - |
dc.citation.volume | 14 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 7608 | - |
dc.citation.endPage | 7615 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.identifier.wosid | A1994PY00600033 | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | METABOTROPIC GLUTAMATE-RECEPTOR | - |
dc.subject.keywordPlus | SYNAPTIC TRANSMISSION | - |
dc.subject.keywordPlus | CA2+ CHANNELS | - |
dc.subject.keywordPlus | N-TYPE | - |
dc.subject.keywordPlus | PRESYNAPTIC INHIBITION | - |
dc.subject.keywordPlus | FUNCTIONAL EXPRESSION | - |
dc.subject.keywordPlus | PHARMACOLOGICAL PROPERTIES | - |
dc.subject.keywordPlus | NEUROTRANSMITTER RELEASE | - |
dc.subject.keywordPlus | HIPPOCAMPAL-NEURONS | - |
dc.subject.keywordPlus | MEDIATED INHIBITION | - |
dc.subject.keywordAuthor | ENKEPHALIN | - |
dc.subject.keywordAuthor | AUTONOMIC NERVOUS SYSTEM | - |
dc.subject.keywordAuthor | MU-OPIOID RECEPTOR | - |
dc.subject.keywordAuthor | OMEGA-CONOTOXIN-GVIA | - |
dc.subject.keywordAuthor | DIHYDROPYRIDINE | - |
dc.subject.keywordAuthor | OMEGA-AGATOXIN-IVA | - |
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