Proteomic Analysis of Cell Wall Proteins with Various Linkages in Fusarium graminearum

Authors
Moon, HeejiMin, KyunghunWinarto, JessicaShin, SoobinJeon, HosungSong, Dae-GeunSon, Hokyoung
Issue Date
2024-03
Publisher
American Chemical Society
Citation
Journal of Agricultural and Food Chemistry, v.72, no.11, pp.6028 - 6039
Abstract
The fungal cell wall, primarily comprising a glucan-chitin matrix and cell wall proteins (CWPs), serves as a key mediator for fungal interactions with the environment and plays a pivotal role in virulence. In this study, we employed a comprehensive proteomics approach to analyze the CWPs in the plant pathogenic fungus Fusarium graminearum. Our methodology successfully extracted and identified 1373 CWPs, highlighting their complex linkages, including noncovalent bonds, disulfide bridges, alkali-sensitive linkages, and glycosylphosphatidylinositol (GPI) anchors. A significant subset of these proteins, enriched in Gene Ontology terms, suggest multifunctional roles of CWPs. Through the integration of transcriptomic and proteomic data, we observed differential expression patterns of CWPs across developmental stages. Specifically, we focused on two genes, Fca7 and Cpd1, which were upregulated in planta, and confirmed their localization predominantly outside the plasma membrane, primarily in the cell wall and periplasmic space. The disruption of FCA7 reduced virulence on wheat, aligning with previous findings and underscoring its significance. Overall, our findings offer a comprehensive proteomic profile of CWPs in F. graminearum, laying the groundwork for a deeper understanding of their roles in the development and interactions with host plants.
Keywords
PATHOGENICITY; WHEAT; IDENTIFICATION; TRANSCRIPTOME; INFECTION; GLYCANS; PATHWAY; DISEASE; TOOL; Fusarium graminearum; cell wall proteins; typeof linkages; proteomics
ISSN
0021-8561
URI
https://pubs.kist.re.kr/handle/201004/149690
DOI
10.1021/acs.jafc.3c07746
Appears in Collections:
KIST Article > 2024
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