Photonic control of ligand nanospacing in self-assembly regulates stem cell fate

Authors
Lee, SungkyuYoo, JounghyunBae, GunhyuThangam, RamarHeo, JeongyunPark, Jung YeonChoi, HonghwanKim, ChowonAn, JusungKim, JungryunMun, Kwang RokShin, SeungyongZhang, KunyuZhao, PengchaoKim, YuriKang, NayeonHan, Seong-BeomKim, DaheeYoon, JiwonKang, MisunKim, JihwanYang, LetaoKaramikamkar, SolmazKim, JinjooZhu, YangzhiNajafabadi, Alireza HassaniSong, GuoshengKim, Dong-HweeLee, Ki-BumOh, Soong JuJung, Hyun-DoSong, Hyun-CheolJang, Woo YoungBian, LimingChu, ZhiqinYoon, JuyoungKim, Jong SeungZhang, Yu ShrikeKim, YongjuJang, Ho SeongKim, SehoonKang, Heemin
Issue Date
2024-04
Publisher
Elsevier
Citation
Bioactive Materials, v.34, pp.164 - 180
Abstract
Extracellular matrix (ECM) undergoes dynamic inflation that dynamically changes ligand nanospacing but has not been explored. Here we utilize ECM-mimicking photocontrolled supramolecular ligand-tunable Azo+ self assembly composed of azobenzene derivatives (Azo+) stacked via cation-pi interactions and stabilized with RGD ligand-bearing poly(acrylic acid). Near-infrared-upconverted-ultraviolet light induces cis-Azo+-mediated inflation that suppresses cation-pi interactions, thereby inflating liganded self-assembly. This inflation increases nanospacing of "closely nanospaced" ligands from 1.8 nm to 2.6 nm and the surface area of liganded self assembly that facilitate stem cell adhesion, mechanosensing, and differentiation both in vitro and in vivo, including the release of loaded molecules by destabilizing water bridges and hydrogen bonds between the Azo+ molecules and loaded molecules. Conversely, visible light induces trans-Azo+ formation that facilitates cation-pi interactions, thereby deflating self-assembly with "closely nanospaced" ligands that inhibits stem cell adhesion, mechanosensing, and differentiation. In stark contrast, when ligand nanospacing increases from 8.7 nm to 12.2 nm via the inflation of self-assembly, the surface area of "distantly nanospaced" ligands increases, thereby suppressing stem cell adhesion, mechanosensing, and differentiation. Long-term in vivo stability of self-assembly via real-time tracking and upconversion are verified. This tuning of ligand nanospacing can unravel dynamic ligand-cell interactions for stem cell-regulated tissue regeneration.
Keywords
HYDROGEL; PI; ADHESION; BEHAVIOR; THERAPY; CYCLE; In vivo tracking; Stem cell adhesion; Stem cell fate; Dynamic self-assembly; Ligand nanospacing
ISSN
2452-199X
URI
https://pubs.kist.re.kr/handle/201004/149977
DOI
10.1016/j.bioactmat.2023.12.011
Appears in Collections:
KIST Article > 2024
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