Osteogenic Activation by hexane fraction of cotoneaster wilsonii Nakai suppresses bone loss in Ovariectomized Mice

Abstract

Osteoporosis, characterized by microstructural depletion in bone tissue and a subsequent decrease in bone density, leading to an increased risk of fractures. Osteoporosis results from imbalance between bone formation and bone resorption with age. Development of osteogenic agents is a current demand to treat osteoporosis. In this study, we evaluated the impact of CWH (hexane fraction of Cotoneaster wilsonii Nakai) on osteogenic differentiation and its therapeutic effect on osteoporosis using an ovariectomized mouse model.In vitro experiments revealed that CWH treatment induced activity of alkaline phosphatase (ALP) in osteoblast differentiation. Also, CWH increased the mRNA and protein expression of key osteogenic differentiation markers, Alkaline phosphatase (ALP), Osteocalcin (OCN) and Osterix (OSX). This confirmed the role of CWH in promoting maturation and differentiation of osteoblasts. Ovariectomy, removal of ovaries, is a major contributor to imbalances and bone loss by elimination of estrogens. In ovariectomized mice, CWH treatment prevents of bone loss and promoted bone tissue regeneration. These effect of CWH was mediated by the induction of osteogenic markers in the bone.