Genetic Ablation of the Conidiogenesis Regulator Enhances Mycoprotein Production

Abstract

Mycoprotein, a filamentous fungi-based protein substitute for traditional meat products, plays a crucial role in ensuring global food security. While genetic manipulation of mycoprotein-producing fungi holds promise for improving key traits, such as higher protein content and biomass yield, substantial progress has yet to be made. In this study, we investigated the function of genes related to conidiogenesis to identify valuable genetic elements for increasing fungal biomass yields in Fusarium venenatum. In the FvFLBD knockout mutant of F. venenatum, fungal biomass increased compared to the wild-type strain, with conidia formation completely abolished. Nutrient profiling further revealed elevated amino acid content in this mutant, likely due to metabolic changes. Additionally, we observed synergistic effects on biomass yield in the FvFLBD and FvUBQ14 double knockout, suggesting that this approach could serve as a comprehensive strategy for enhancing fungal food production. Our findings provide valuable genetic insights and broaden the horizon of mycoprotein applications in the food industry.