Synchronous diagnosis of respiratory viruses variants via receptonics based on modeling receptor-ligand dynamics

Abstract

The transmission and pathogenesis of highly contagious fatal respiratory viruses are increasing, and the need for an on-site diagnostic platform has arisen as an issue worldwide. Furthermore, as the spread of respiratory virus continues, different variants have become the dominant circulating strains. To prevent virus transmission, the development of highly sensitive and accurate on-site diagnostic assays is urgently needed. Herein, we present a facile diagnostic device for multi-detection based on the results of detailed receptor？ligand dynamics simulations for the screening of various viral strains. Our novel bioreceptor？treated electronics (receptonics) device consists of a multichannel graphene transistor and cell-entry receptors conjugated to N-heterocyclic carbene (NHC). We achieved ultrasensitive multi-detection performance without the need for sample pretreatment, which will enable rapid diagnosis and prevent the spread of pathogens. This platform can be applied for the diagnosis of variants of concern in clinical respiratory virus samples and primate models. Our multi-screening platform can be used to enhance surveillance and discriminate emerging virus variants before they become a severe threat to public health.