SARS-CoV-2 Variant Screening Using a Virus-Receptor-Based Electrical Biosensor

Authors
Park, SungwookKim, HojunWoo KyoungminKim, Jeong-MinJo, Hye-JunJeong, YoungdoLee, Kwan Hyi
Issue Date
2022-01
Publisher
American Chemical Society
Citation
Nano Letters, v.22, no.1, pp.50 - 57
Abstract
SARS-CoV-2 variants are of particular interest because they can potentially increase the transmissibility and virulence of COVID-19 or reduce the effectiveness of available vaccines. However, screening SARS-CoV-2 variants is a challenge because biosensors target viral components that can mutate. One promising strategy is to screen variants via angiotensin-converting enzyme 2 (ACE2), a virus receptor shared by all known SARS-CoV-2 variants. Here we designed a highly sensitive and portable COVID-19 screening biosensor based on the virus receptor. We chose a dualgate field-effect transistor to overcome the low sensitivity of virusreceptor-based biosensors. To optimize the biosensor, we introduced a synthetic virus that mimics the important features of SARS-CoV-2 (size, bilayer structure, and composition). The developed biosensor successfully detected SARS-CoV-2 in 20 min and showed sensitivity comparable to that of molecular diagnostic tests (similar to 165 copies/ mL). Our results indicate that a virus-receptor-based biosensor can be an effective strategy for screening infectious diseases to prevent pandemics.
Keywords
CORONAVIRUS; COVID-19; SPIKE; DIAGNOSIS; SENSOR; SARS-CoV-2; variant screening; ACE2; virus receptor; biosensor
ISSN
1530-6984
URI
https://pubs.kist.re.kr/handle/201004/115841
DOI
10.1021/acs.nanolett.1c03108
Appears in Collections:
KIST Article > 2022
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