Prunetin 4′-o-phosphate, a novel compound, in raw 264.7 macrophages exerts anti-inflammatory activity via suppression of map kinases and the nfκb pathway

Authors
Park, T.-J.Hong, H.Kim, M.-S.Park, J.-S.Chi, W.-J.Kim, S.-Y.
Issue Date
2021-11
Publisher
MDPI
Citation
Molecules, v.26, no.22
Abstract
Biorenovation, a microbial enzyme-assisted degradation process of precursor compounds, is an effective approach to unraveling the potential bioactive properties of the derived compounds. In this study, we obtained a new compound, prunetin 4′-O-phosphate (P4P), through the biorenovation of prunetin (PRN), and investigated its anti-inflammatory effects in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells. The anti-inflammatory effect of P4P was evaluated by measuring the production of prostaglandin-E2 (PGE2), nitric oxide (NO), which is an inflammation-inducing factor, and related cytokines such as tumor necrosis factor-α (TNFα), interleukin-1β (IL1β), and interleukin-6 (IL6). The findings demonstrated that P4P was non-toxic to cells, and its inhibition of the secretion of NO―as well as pro-inflammatory cytokines―was concentration-dependent. A simultaneous reduction in the protein expression level of pro-inflammatory proteins such as cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) was observed. Moreover, the phosphorylation of mitogen-activated protein kinases (MAPKs) such as extracellular signal-regulated kinases (ERKs), c-Jun N-terminal kinase (JNK), p38 MAPK (p38), and nuclear factor kappa B (NFκB) was downregulated. To conclude, we report that biorenovation-based phosphorylation of PRN improved its anti-inflammatory activity. Cell-based in vitro assays further confirmed that P4P could be applied in the development of anti-inflammatory therapeutics. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Keywords
SIGNALING PATHWAY; MOLECULAR-MECHANISMS; GENE-EXPRESSION; IN-VITRO; INHIBITION; INFLAMMATION; ACTIVATION; GENISTEIN; INNATE; AGENTS; Anti-inflammatory activities; Biorenovation; MAPK signaling; Phosphorylation; Prunetin; Prunetin 4′-O-phosphate
ISSN
1420-3049
URI
https://pubs.kist.re.kr/handle/201004/116197
DOI
10.3390/molecules26226841
Appears in Collections:
KIST Article > 2021
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