In vitro chondrocyte culture using hydrophilized PLLA scaffold in bioreactor system
- Authors
- Yang, Hee Seok; Park, Kwideok; Kim, Byoung-Soo; Kim, Jae-Jin; Ahn, Kwang-Duk; Han, Dong Keun
- Issue Date
- 2007
- Publisher
- TRANS TECH PUBLICATIONS LTD
- Citation
- 7th Asian Symposium on Biomedical Materials (ASBM7), v.342-343, pp.253 - +
- Abstract
- Nonporous PLLA film and porous PLLA scaffolds were prepared and then grafted with acrylic acid (AA) using in situ direct plasma treatment to obtain PLLA-g-PAA. Chondrocytes isolated from rabbit knee articular cartilages were cultivated in Dulbecco's modified eagle medium-F12 (DMEM-F12) containing 10% fetal bovine serum (FBS) and 1% antibiotics and passaged twice before cell seeding. Once seeded on either PLLA films or scaffolds, they were placed in a bioreactor system and an intermittent hydrodynamic pressure (IHP) was applied in 3 bars, while turned on for 2 min and off for 28 min during 15-day culture. AA grafting to PLLA surface was confirmed from various surface analyses. From WST-1 assay, chondrocyte proliferation was significantly improved with dynamic IHP for PLLA and PLLA-g-PAA scaffolds as compared to static culture. This study indicates that IHP may have significant influence on chondrocytes behavior in 3D culture environment.
- ISSN
- 1013-9826
- URI
- https://pubs.kist.re.kr/handle/201004/116437
- DOI
- 10.4028/www.scientific.net/KEM.342-343.253
- Appears in Collections:
- KIST Conference Paper > 2007
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