Evolution of CRISPR towards accurate and efficient mammal genome engineering

Authors
Ryu, Seuk-MinHur, Junseok W.Kim, Kyoungmi
Issue Date
2019-08
Publisher
생화학분자생물학회
Citation
BMB Reports, v.52, no.8, pp.475 - 481
Abstract
The evolution of genome editing technology based on CRISPR (clustered regularly interspaced short palindromic repeats) system has led to a paradigm shift in biological research. CRISPR/Cas9-guide RNA complexes enable rapid and efficient genome editing in mammalian cells. This system induces double-stranded DNA breaks (DSBs) at target sites and most DNA breakages induce mutations as small insertions or deletions (indels) by non-homologous end joining (NHEJ) repair pathway. However, for more precise correction as knock-in or replacement of DNA base pairs, using the homology-directed repair (HDR) pathway is essential. Until now, many trials have greatly enhanced knock-in or substitution efficiency by increasing HDR efficiency, or newly developed methods such as Base Editors (BEs). However, accuracy remains unsatisfactory. In this review, we summarize studies to overcome the limitations of HDR using the CRISPR system and discuss future direction.
Keywords
STRAND BREAK REPAIR; HOMOLOGY-DIRECTED REPAIR; ONE-STEP GENERATION; DNA-REPAIR; KNOCK-IN; BASE; PATHWAY; RECOMBINATION; INHIBITION; PRECISION; CRISPR; DNA double-strand break; Genome editing; HDR; NHEJ
ISSN
1976-6696
URI
https://pubs.kist.re.kr/handle/201004/119698
DOI
10.5483/BMBRep.2019.52.8.149
Appears in Collections:
KIST Article > 2019
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE