Eupatilin inhibits angiogenesis-mediated human hepatocellular metastasis by reducing MMP-2 and VEGF signaling
- Authors
- Park, Jun Yeon; Park, Do Hwi; Jeon, Youngsic; Kim, Young-Joo; Lee, Jaemin; Shin, Myoung-Sook; Kang, Ki Sung; Hwang, Gwi Seo; Kim, Hyun Young; Yamabe, Noriko
- Issue Date
- 2018-10-15
- Publisher
- Pergamon Press Ltd.
- Citation
- Bioorganic & Medicinal Chemistry Letters, v.28, no.19, pp.3150 - 3154
- Abstract
- Metastasis is responsible for the great majority of deaths in cancer patients. Matrix metalloproteinases (MMPs) have critical functions in cancer metastasis. Especially, MMP-2 and MMP-9 play a major role in tumor-cell migration and invasion. Therefore, to first find out the inhibitory effect of eupatilin on expression of MMPs in SNU182 cells, we used quantitative real-rime PCR to measure MMP-2 and MMP-9 mRNA levels. Eupatilin suppressed transcription of MMP-2 in SNU182 cells more than did the corresponding controls. Also, eupatilin significantly blocked tube formation when treated with a concentration of 3.125 or 6.25 mu g/mL on human umbilical vein vascular endothelial cells (HUVECs). Eupatilin induced significant anti-angiogenic potential associated with down-regulation of hypoxia-inducible factor 1-alpha (HIF-1 alpha), vascular endothelial growth factor (VEGF), and phosphorylated Akt expression. Thus, tube-formation inhibition and MMP-2-mediated migration are likely to be important therapeutic targets of eupatilin in hepatocellular carcinoma metastasis.
- Keywords
- NITRIC-OXIDE SYNTHASE; MATRIX METALLOPROTEINASES; EXPRESSION; CARCINOMA; PATHOGENESIS; COLLAGENASE; MIGRATION; EXTRACT; CELLS; ACID; NITRIC-OXIDE SYNTHASE; MATRIX METALLOPROTEINASES; EXPRESSION; CARCINOMA; PATHOGENESIS; COLLAGENASE; MIGRATION; EXTRACT; CELLS; ACID; Eupatilin; Metastasis; HUVEC; MMPs; VEGF
- ISSN
- 0960-894X
- URI
- https://pubs.kist.re.kr/handle/201004/120784
- DOI
- 10.1016/j.bmcl.2018.08.034
- Appears in Collections:
- KIST Article > 2018
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