Dimeric Human β-Defensin 3 as a Universal Platform for Intracellular Delivery of Nucleic Acid Cargos

Authors
Kim, H.Y.Jang, J.-E.Ahn, D.-R.
Issue Date
2018-07
Publisher
American Chemical Society
Citation
ACS Applied Bio Materials, v.1, no.1, pp.100 - 109
Abstract
Functional nucleic acids including siRNA, mRNA, and plasmid DNA are promising bioactive molecules to regulate cellular functions uncontrollable by conventional small molecule regulators. To realize successful cellular applications of these nucleic acids, an intracellular gene delivery vehicle with high efficiency and low cytotoxicity is required. Here, we report the dimerization of human β-defensin 3 (DhBD3) promoted by the interaction between β-strands and the application of DhBD3 for efficient delivery of various nucleic acid cargos. DhBD3 with multiple cationic residues could be complexed with various types of polyanionic DNA and RNA. DhBD3 could intracellularly deliver both small and large nucleic acid cargos loaded by complexation to regulate the expression level of target proteins, showing its potential as a universal platform for nucleic acid delivery. In addition, as DhBD3 is a human-derived material with high biocompatibility and can be robustly prepared by an inexpensive method, it is a promising gene delivery system that can be employed for biomedical purposes. ? 2018 American Chemical Society.
Keywords
Biocompatibility; Biomolecules; Gene transfer; Genes; Cellular applications; Defensins; Functional nucleic acids; Gene Delivery; Intracellular delivery; Intracellular gene delivery; Nucleic acid deliveries; Self aggregation; Nucleic acids; Biocompatibility; Biomolecules; Gene transfer; Genes; Cellular applications; Defensins; Functional nucleic acids; Gene Delivery; Intracellular delivery; Intracellular gene delivery; Nucleic acid deliveries; Self aggregation; Nucleic acids; dimeric β-defensin 3; gene delivery vehicles; nucleic acid cargos; self-aggregation; β-defensin
ISSN
2576-6422
URI
https://pubs.kist.re.kr/handle/201004/121230
DOI
10.1021/acsabm.8b00024
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KIST Article > 2018
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