Ginsenoside Rg1 from Panax ginseng enhances myoblast differentiation and myotube growth

Authors
Go, Ga-YeonLee, Sang-JinJo, AyoungLee, JaecheolSeo, Dong-WanKang, Jong-SunKim, Si-KwanKim, Su-NamKim, Yong KeeBae, Gyu-Un
Issue Date
2017-10
Publisher
KOREAN SOC GINSENG
Citation
JOURNAL OF GINSENG RESEARCH, v.41, no.4, pp.608 - 614
Abstract
Background: Ginsenoside Rg1 belongs to protopanaxatriol-type ginsenosides and has diverse pharmacological activities. In this report, we investigated whether Rg1 could upregulate muscular stem cell differentiation and muscle growth. Methods: C2C12 myoblasts, MyoD-transfected 10T1/2 embryonic fibroblasts, and HEK293T cells were treated with Rg1 and differentiated for 2 d, subjected to immunoblotting, immunocytochemistry, or immunoprecipitation. Results: Rg1 activated promyogenic kinases, p38MAPK (mitogen-activated protein kinase) and Akt signaling, that in turn promote the heterodimerization with MyoD and E proteins, resulting in enhancing myogenic differentiation. Through the activation of Akt/mammalian target of rapamycin pathway, Rg1 induced myotube growth and prevented dexamethasone-induced myotube atrophy. Furthermore, Rg1 increased MyoD-dependent myogenic conversion of fibroblast. Conclusion: Rg1 upregulates promyogenic kinases, especially Akt, resulting in improvement of myoblast differentiation and myotube growth. (C) 2017 The Korean Society of Ginseng, Published by Elsevier Korea LLC.
Keywords
ACTIVATED PROTEIN-KINASE; SKELETAL-MUSCLE DIFFERENTIATION; FOXO TRANSCRIPTION FACTORS; PATHWAY; CELL; HYPERTROPHY; MYOGENESIS; ATROPHY; CDO; EXPRESSION; ACTIVATED PROTEIN-KINASE; SKELETAL-MUSCLE DIFFERENTIATION; FOXO TRANSCRIPTION FACTORS; PATHWAY; CELL; HYPERTROPHY; MYOGENESIS; ATROPHY; CDO; EXPRESSION; ginsenoside Rg1; myoblast differentiation; myogenic conversion; myotube hypertrophy; promyogenic signaling
ISSN
1226-8453
URI
https://pubs.kist.re.kr/handle/201004/122245
DOI
10.1016/j.jgr.2017.05.006
Appears in Collections:
KIST Article > 2017
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