Perspectives for biocatalytic lignin utilization: cleaving 4-O-5 and C-alpha-C-beta bonds in dimeric lignin model compounds catalyzed by a promiscuous activity of tyrosinase

Authors
Min Kyoung SeonYum, tae wooKim JiyeWoo, Han MinKim, YunjeSang, Byoung-InYoo, Young JeKim, Yong HwanUm, Youngsoon
Issue Date
2017-09
Publisher
BioMed Central
Citation
Biotechnology for Biofuels, v.10
Abstract
Background: In the biorefinery utilizing lignocellulosic biomasses, lignin decomposition to value-added phenolic derivatives is a key issue, and recently biocatalytic delignification is emerging owing to its superior selectivity, low energy consumption, and unparalleled sustainability. However, besides heme-containing peroxidases and laccases, information about lignolytic biocatalysts is still limited till date. Results: Herein, we report a promiscuous activity of tyrosinase which is closely associated with delignification requiring high redox potentials (>1.4 V vs. normal hydrogen electrode [NHE]). The promiscuous activity of tyrosinase not only oxidizes veratryl alcohol, a commonly used nonphenolic substrate for assaying ligninolytic activity, to veratraldehyde but also cleaves the 4-O-5 and C-alpha-C-beta bonds in 4-phenoxyphenol and guaiacyl glycerol-beta-guaiacyl ether (GGE) that are dimeric lignin model compounds. Cyclic voltammograms additionally verified that the promiscuous activity oxidizes lignin-related high redox potential substrates. Conclusion: These results might be applicable for extending the versatility of tyrosinase toward biocatalytic delignification as well as suggesting a new perspective for sustainable lignin utilization. Furthermore, the results provide insight for exploring the previously unknown promiscuous activities of biocatalysts much more diverse than ever thought before, thereby innovatively expanding the applicable area of biocatalysis.
Keywords
SITE-DIRECTED MUTAGENESIS; RHODOCOCCUS-JOSTII RHA1; AGARICUS-BISPORUS; L-DOPA; PEROXIDASE; OXIDATION; ENZYMES; DEGRADATION; SPECIFICITY; PATHWAYS; Sustainable lignin utilization; Tyrosinase; Promiscuous activity; 4-Phenoxyphenol; Guaiacyl glycerol-beta-guaiacyl ether (GGE)
ISSN
1754-6834
URI
https://pubs.kist.re.kr/handle/201004/122327
DOI
10.1186/s13068-017-0900-3
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KIST Article > 2017
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