Rational design of a photo-crosslinking BODIPY for in situ protein labeling
- Authors
- Murale, Dhiraj P.; Hong, Seong Cheol; Yun, JiHyeon; Yoon, Chang No; Lee, Jun-Seok
- Issue Date
- 2015-04
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- CHEMICAL COMMUNICATIONS, v.51, no.30, pp.6643 - 6646
- Abstract
- Photo-crosslinking agents have emerged as critical tools to investigate protein-protein interactions in complex proteomes, but there are few photocrosslinkers available at the moment. Here, we report the first rational design of a photo-crosslinking BODIPY fluorophore (pcBD) and its biological application for biomolecule labeling. As a photo-sensitizing functional motif, an aryl ketone group was incorporated into the BODIPY fluorophore, and a series of proteins were labeled by pcBD compounds upon UV irradiation. In order to investigate protein-protein interactions in a protein mixture, amino-functionalized pcBD was prepared and covalently attached to a ubiquitin ligase binding peptide. Upon UV irradiation, we could successfully visualize the substrates in the total lysate. These results provided a proof of concept for spatially controllable tagging via photo-activation of the pcBD scaffold, and demonstrated its potential usage for in situ labeling applications.
- Keywords
- LIVING CELLS; LIVING CELLS; fluorescence; BODIPY; protein labeling
- ISSN
- 1359-7345
- URI
- https://pubs.kist.re.kr/handle/201004/125622
- DOI
- 10.1039/c5cc01068c
- Appears in Collections:
- KIST Article > 2015
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