Fast spore breaking by superheating

Authors
Pribylka, AdamAlmeida, Ana VanessaAltmeyer, Matthias O.Petr, JanSevcik, JurajManz, AndreasNeuzil, Pavel
Issue Date
2013-05
Publisher
Royal Society of Chemistry
Citation
Lab on a Chip, v.13, no.9, pp.1695 - 1698
Abstract
Here we present the results of Bacillus subtilis spores breaking using superheating. The spore sample was pumped through the open-ended capillary tube mounted across the heated zone. We investigated the influence of temperature in the range 120-180 degrees C. The heat exposure was controlled by the length of the heated zone, the inner diameter of the capillary and the sample flow rate. We found that spore treatment above 120 degrees C resulted in the release of DNA within 20 s.
Keywords
SAMPLE PREPARATION METHOD; BACTERIAL-SPORES; BACILLUS-SUBTILIS; ANTHRAX SPORES; DNA ANALYSIS; RESISTANCE; CELLS; HEAT; GERMINATION; DISRUPTION; SAMPLE PREPARATION METHOD; BACTERIAL-SPORES; BACILLUS-SUBTILIS; ANTHRAX SPORES; DNA ANALYSIS; RESISTANCE; CELLS; HEAT; GERMINATION; DISRUPTION
ISSN
1473-0197
URI
https://pubs.kist.re.kr/handle/201004/128086
DOI
10.1039/c3lc41305e
Appears in Collections:
KIST Article > 2013
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