Fast spore breaking by superheating
- Authors
- Pribylka, Adam; Almeida, Ana Vanessa; Altmeyer, Matthias O.; Petr, Jan; Sevcik, Juraj; Manz, Andreas; Neuzil, Pavel
- Issue Date
- 2013-05
- Publisher
- Royal Society of Chemistry
- Citation
- Lab on a Chip, v.13, no.9, pp.1695 - 1698
- Abstract
- Here we present the results of Bacillus subtilis spores breaking using superheating. The spore sample was pumped through the open-ended capillary tube mounted across the heated zone. We investigated the influence of temperature in the range 120-180 degrees C. The heat exposure was controlled by the length of the heated zone, the inner diameter of the capillary and the sample flow rate. We found that spore treatment above 120 degrees C resulted in the release of DNA within 20 s.
- Keywords
- SAMPLE PREPARATION METHOD; BACTERIAL-SPORES; BACILLUS-SUBTILIS; ANTHRAX SPORES; DNA ANALYSIS; RESISTANCE; CELLS; HEAT; GERMINATION; DISRUPTION; SAMPLE PREPARATION METHOD; BACTERIAL-SPORES; BACILLUS-SUBTILIS; ANTHRAX SPORES; DNA ANALYSIS; RESISTANCE; CELLS; HEAT; GERMINATION; DISRUPTION
- ISSN
- 1473-0197
- URI
- https://pubs.kist.re.kr/handle/201004/128086
- DOI
- 10.1039/c3lc41305e
- Appears in Collections:
- KIST Article > 2013
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