Ethanol-Dispersed Polymer Nanofibers as a Highly Selective Cell Isolation and Release Platform for CD4(+) T Lymphocytes

Authors
Jun, Seung-HyunKim, KwangheeAn, Hyo JinKim, Byoung ChanSonn, Chung HeeKim, MijuDoh, JunsangYee, CassianLee, Kyung-MiKim, Jungbae
Issue Date
2012-11-07
Publisher
WILEY-V C H VERLAG GMBH
Citation
ADVANCED FUNCTIONAL MATERIALS, v.22, no.21, pp.4448 - 4455
Abstract
A novel cell isolation and release platform using electrospun polystyrene-poly(styrene-co-maleic anhydride) (PS-PSMA) nanofibers is presented. Ethanol treatment of PS-PSMA nanofibers, employed for the purpose of sterilization, significantly increases their inter-fiber space for both antibody conjugation and subsequent cell separation. For the selective isolation of CD4(+) T cells from heterogeneous mixtures of mouse lymph nodes, capture efficiencies of up to 100% are achieved while maintaining cellular integrity and viability. Once captured, CD4(+) T lymphocytes can also be released from the NF scaffolds, further demonstrating its potential functionality as an immune cell-supporting and releasing matrix. This is the first demonstration of lymphocyte-culture scaffolds enabling selective isolation, accommodation, and sustained release of CD4(+) T cells for the purpose of cell therapies.
Keywords
PANCREATIC-CANCER; PROTEIN; ELECTROSPUN; DELIVERY; COATINGS; HER2/NEU; PANCREATIC-CANCER; PROTEIN; ELECTROSPUN; DELIVERY; COATINGS; HER2/NEU; electrospun polymer nanofibers; ethanol dispersion; CD4+T lymphocyte; cell separation; cell transplantation
ISSN
1616-301X
URI
https://pubs.kist.re.kr/handle/201004/128656
DOI
10.1002/adfm.201200657
Appears in Collections:
KIST Article > 2012
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