Ethanol-Dispersed Polymer Nanofibers as a Highly Selective Cell Isolation and Release Platform for CD4(+) T Lymphocytes
- Authors
- Jun, Seung-Hyun; Kim, Kwanghee; An, Hyo Jin; Kim, Byoung Chan; Sonn, Chung Hee; Kim, Miju; Doh, Junsang; Yee, Cassian; Lee, Kyung-Mi; Kim, Jungbae
- Issue Date
- 2012-11-07
- Publisher
- WILEY-V C H VERLAG GMBH
- Citation
- ADVANCED FUNCTIONAL MATERIALS, v.22, no.21, pp.4448 - 4455
- Abstract
- A novel cell isolation and release platform using electrospun polystyrene-poly(styrene-co-maleic anhydride) (PS-PSMA) nanofibers is presented. Ethanol treatment of PS-PSMA nanofibers, employed for the purpose of sterilization, significantly increases their inter-fiber space for both antibody conjugation and subsequent cell separation. For the selective isolation of CD4(+) T cells from heterogeneous mixtures of mouse lymph nodes, capture efficiencies of up to 100% are achieved while maintaining cellular integrity and viability. Once captured, CD4(+) T lymphocytes can also be released from the NF scaffolds, further demonstrating its potential functionality as an immune cell-supporting and releasing matrix. This is the first demonstration of lymphocyte-culture scaffolds enabling selective isolation, accommodation, and sustained release of CD4(+) T cells for the purpose of cell therapies.
- Keywords
- PANCREATIC-CANCER; PROTEIN; ELECTROSPUN; DELIVERY; COATINGS; HER2/NEU; PANCREATIC-CANCER; PROTEIN; ELECTROSPUN; DELIVERY; COATINGS; HER2/NEU; electrospun polymer nanofibers; ethanol dispersion; CD4+T lymphocyte; cell separation; cell transplantation
- ISSN
- 1616-301X
- URI
- https://pubs.kist.re.kr/handle/201004/128656
- DOI
- 10.1002/adfm.201200657
- Appears in Collections:
- KIST Article > 2012
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