A Quantitative Method for Simultaneous Determination of 5-Methoxy-N,N-Diisopropyltryptamine and its Metabolites in Urine Using Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry

Authors
Jin, Ming J.Jin, ChangbaeKim, Jin Y.In, Moon K.Kwon, Oh S.Yoo, Hye H.
Issue Date
2011-07
Publisher
WILEY
Citation
JOURNAL OF FORENSIC SCIENCES, v.56, no.4, pp.1044 - 1048
Abstract
5-Methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT) is a designer hallucinogen derived from tryptamine and is reportedly abused and involved in criminal activities. For the detection of 5-MeO-DIPT use, a liquid chromatography-tandem mass spectrometric method for 5-MeO-DIPT and its metabolites, 5-hydroxy-N,N-diisopropyltryptamine (5-OH-DIPT) and 5-methoxy-N,N-isopropyltryptamine (5-MeO-IPT) was developed and validated in rat urine. The urine samples were pretreated by protein precipitation with acetonitrile and introduced into a BDS HYPERSIL C-18 column (50 x 2.0 mm, 5 mu m) for chromatographic separation. Mobile phases consisted of methanol, water, and 1% formic acid, and gradient elution was used at a flow rate of 0.2 mL/min. For the MS detection, multiple-reaction monitoring analysis was adopted. The linear range was 0.01-10 mu g/mL, and the lower limit of quantification was 10 ng/mL for all analytes. The intra- and interday accuracies and precisions met the criteria (< 15%). The developed method was successfully applied to the drug-treated rat urine.
Keywords
LIVER-MICROSOMES; N,N-DIISOPROPYLTRYPTAMINE; FOXY; forensic science; 5-MeO-DIPT; 5-OH-DIPT; 5-MeO-IPT; urine; LC/MS/MS
ISSN
0022-1198
URI
https://pubs.kist.re.kr/handle/201004/130207
DOI
10.1111/j.1556-4029.2011.01753.x
Appears in Collections:
KIST Article > 2011
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