Cholecystokinin Facilitates Glutamate Release by Increasing the Number of Readily Releasable Vesicles and Releasing Probability

Authors
Deng, Pan-YueXiao, ZhaoyangJha, ArchanaRamonet, DavidMatsui, ToshimitsuLeitges, MichaelShin, Hee-SupPorter, James E.Geiger, Jonathan D.Lei, Saobo
Issue Date
2010-04-14
Publisher
SOC NEUROSCIENCE
Citation
JOURNAL OF NEUROSCIENCE, v.30, no.15, pp.5136 - 5148
Abstract
Cholecystokinin (CCK), a neuropeptide originally discovered in the gastrointestinal tract, is abundantly distributed in the mammalian brains including the hippocampus. Whereas CCK has been shown to increase glutamate concentration in the perfusate of hippocampal slices and in purified rat hippocampal synaptosomes, the cellular and molecular mechanisms whereby CCK modulates glutamatergic function remain unexplored. Here, we examined the effects of CCK on glutamatergic transmission in the hippocampus using whole-cell recordings from hippocampal slices. Application of CCK increased AMPA receptor-mediated EPSCs at perforant path-dentate gyrus granule cell, CA3-CA3 and Schaffer collateral-CA1 synapses without effects at mossy fiber-CA3 synapses. CCK-induced increases in AMPA EPSCs were mediated by CCK-2 receptors and were not modulated developmentally and transcriptionally. CCK reduced the coefficient of variation and paired-pulse ratio of AMPA EPSCs suggesting that CCK facilitates presynaptic glutamate release. CCK increased the release probability and the number of readily releasable vesicles with no effects on the rate of recovery from vesicle depletion. CCK-mediated increases in glutamate release required the functions of phospholipase C, intracellular Ca2+ release and protein kinase C gamma. CCK released endogenously from hippocampal interneurons facilitated glutamatergic transmission. Our results provide a cellular and molecular mechanism to explain the roles of CCK in the brain.
Keywords
INHIBITS NEURONAL EXCITABILITY; RAT HIPPOCAMPAL-NEURONS; ENTORHINAL CORTEX; DENTATE GYRUS; IN-VIVO; PYRAMIDAL NEURONS; GABA RELEASE; K+ CHANNELS; GABAERGIC TRANSMISSION; RECEPTOR LIGANDS; INHIBITS NEURONAL EXCITABILITY; RAT HIPPOCAMPAL-NEURONS; ENTORHINAL CORTEX; DENTATE GYRUS; IN-VIVO; PYRAMIDAL NEURONS; GABA RELEASE; K+ CHANNELS; GABAERGIC TRANSMISSION; RECEPTOR LIGANDS
ISSN
0270-6474
URI
https://pubs.kist.re.kr/handle/201004/131543
DOI
10.1523/JNEUROSCI.5711-09.2010
Appears in Collections:
KIST Article > 2010
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE