Apatite-coated poly(lactic-co-glycolic acid) microspheres as an injectable scaffold for bone tissue engineering

Authors
Kang, Sun-WoongYang, Hee SeokSeo, Sang-WooHan, Dong KeunKim, Byung-Soo
Issue Date
2008-06-01
Publisher
WILEY
Citation
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, v.85A, no.3, pp.747 - 756
Abstract
Biodegradable polymer/ceramic composite scaffold could overcome limitations of biodegradable polymers or ceramics for bone regeneration. Injectable scaffold has raised great interest for bone regeneration in vivo, since it allows one for easy filling of irregularly shaped bone defects and implantation of osteogenic cells through minimally invasive surgical procedures The purpose of this study was to determine whether apatite-coated poly(lactic-co-glycolic acid) (PLGA) microspheres could be used as an injectable scaffold to regenerate bone in vivo. Apatite-coated PLGA microspheres were fabricated by incubating PLGA microspheres in simulated body fluid. The apatite that coated the PLGA microsphere surfaces was similar to apatite in natural bone, as demonstrated by scanning electron microscopy, X-ray diffraction spectra, energy-dispersive spectroscopy, and Fourier transformed-infrared spectroscopy analyses. Rat osteoblasts were mixed with apatite-coated PLGA microspheres and injected immediately into subcutaneous sites of athymic mice. Osteoblast transplantation with plain PLGA microspheres served as a control. Histological analysis of the implants at 6 weeks with hematoxylin and eosin staining, Masson's trichrome staining, and von Kossa staining revealed much better regeneration of bone in the apatite-coated PLGA microsphere group than the plain PLGA microsphere group. The new bone formation area and the calcium content of the implants were significantly higher in the apatite-coated PLGA microsphere group than in the plain PLGA microsphere group. This study demonstrates the feasibility of using apatite-coated PLGA microspheres as an injectable scaffold for in vivo bone tissue engineering. This scaffold may be useful for bone regeneration through minimally invasive surgical procedures in orthopedic applications. (C) 2007 Wiley Periodicals, Inc.
Keywords
POTENTIAL BULKING AGENT; COMPOSITE SCAFFOLDS; IN-VITRO; OSTEOBLAST ADHESION; NANOPHASE CERAMICS; BIOMIMETIC PROCESS; CELL ATTACHMENT; GLASS-CERAMICS; REGENERATION; CHONDROCYTES; POTENTIAL BULKING AGENT; COMPOSITE SCAFFOLDS; IN-VITRO; OSTEOBLAST ADHESION; NANOPHASE CERAMICS; BIOMIMETIC PROCESS; CELL ATTACHMENT; GLASS-CERAMICS; REGENERATION; CHONDROCYTES; bone regeneration; biodegradable polymer; polymer/ceramic composite; injectable scaffold; tissue engineering
ISSN
1549-3296
URI
https://pubs.kist.re.kr/handle/201004/133400
DOI
10.1002/jbm.a.31572
Appears in Collections:
KIST Article > 2008
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE