Construction of bacterial artificial chromosome library from electrochemical microorganisms

Authors
Back, JHKim, MSCho, HChang, ISLee, JYKim, KSKim, BHPark, YIHan, YS
Issue Date
2004-09-01
Publisher
OXFORD UNIV PRESS
Citation
FEMS MICROBIOLOGY LETTERS, v.238, no.1, pp.65 - 70
Abstract
A microbial fuel cell is a device that directly converts metabolic energy into electricity, using electrochemical technology. The analysis of large genome fragments recovered directly from microbial communities represents one promising approach to characterizing uncultivated electrochemical microorganisms. To further assess the utility of this approach, we constructed large-insert (140 kb) bacterial artificial chromosome (BAC) libraries from the genomic DNA of a microbial fuel cell, which had been operated for three weeks using acetate media. We screened the expression of several ferric reductase activities in the Escherichia coli host, in order to determine the extent of heterologous expression of metal-ion-reducing enzymes in the library. Phylogenetic analysis of 16S rRNA gene sequences recovered from the BAC libraries indicates that they contain DNA from a wide diversity of microbial organisms. The constructed bacterial library proved a powerful tool for exploring metal-ion reductase activities, providing information on the electron transport pathway of electrochemical microbial (ECM) organisms. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Keywords
SHEWANELLA-PUTREFACIENS MR-1; MICROBIAL FUEL-CELL; ESCHERICHIA-COLI; IRON; CYTOCHROME; REDUCTION; ENVIRONMENTS; EXPRESSION; MANGANESE; SHEWANELLA-PUTREFACIENS MR-1; MICROBIAL FUEL-CELL; ESCHERICHIA-COLI; IRON; CYTOCHROME; REDUCTION; ENVIRONMENTS; EXPRESSION; MANGANESE; electrochemical microbial organisms; bacterial artificial chromosome library; microbial fuel cell; metal reductase
ISSN
0378-1097
URI
https://pubs.kist.re.kr/handle/201004/137241
DOI
10.1016/j.femsle.2004.07.018
Appears in Collections:
KIST Article > 2004
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