Cerebellar slice cultures from mice lacking the P/Q calcium channel: electroresponsiveness of Purkinje cells

Authors
Cavelier, PBeekenkamp, HShin, HSJun, KBossu, JL
Issue Date
2002-11-15
Publisher
ELSEVIER SCI IRELAND LTD
Citation
NEUROSCIENCE LETTERS, v.333, no.1, pp.64 - 68
Abstract
To investigate the role of P/Q type Ca2+ channels in determining the firing pattern of Purkinje cells (PCs) we compared the somatically evoked discharge of action potentials (APs) in PCs from 3 to 4 week old cerebellar slice cultures obtained with ataxic mice lacking alpha(1A)-subunit (alpha(-/-)) and with normal mice (non-ataxic alpha(+/-) or alpha(+/+) using the whole-cell configuration of the patch-clamp recording method. Whereas evoked responses of PCs in normal mice were mainly fast APs, those of PCs from ataxic mice were mainly low-threshold Ca2+ spikes (LTS). Furthermore, a sustained plateau potential due to the activation of cadmium sensitive Ca2+ conductances was not observed in PCs from ataxic mice by blocking K+ channels. These results confirm that P/Q Ca2+ Channels elicit Ca2+-dependent plateau potentials and control the propagation of the dendritic LTS to the soma. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
Keywords
ELECTRO-PHYSIOLOGICAL PROPERTIES; LEANER MUTANT MICE; CURRENTS; INVITRO; INITIATION; DENDRITES; ATAXIA; ELECTRO-PHYSIOLOGICAL PROPERTIES; LEANER MUTANT MICE; CURRENTS; INVITRO; INITIATION; DENDRITES; ATAXIA; calcium channels; alpha(1A)-null mutation; excitability; action potential; Purkinje neurons; cerebellum
ISSN
0304-3940
URI
https://pubs.kist.re.kr/handle/201004/139046
DOI
10.1016/S0304-3940(02)00962-X
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KIST Article > 2002
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