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dc.contributor.authorSang, Jun Sim-
dc.contributor.authorSnell, KD-
dc.contributor.authorKim, BW-
dc.contributor.authorRha, CK-
dc.contributor.authorSinskey, AJ-
dc.date.accessioned2024-01-21T11:15:02Z-
dc.date.available2024-01-21T11:15:02Z-
dc.date.created2022-04-05-
dc.date.issued2001-12-
dc.identifier.issn0141-5492-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/139923-
dc.description.abstractA second promoter (P1) was inserted to the PHA (poly-beta-hydroxyalkanoate) operon (pSP2) of Esherichia coli DH5alpha with an optimal E. coli ribosome binding site and a trc strong promoter (pSJS1) to obtain poly-beta-hydroxybutyrate (PHB) with long chain length. When the inducer, IPTG was added to the culture at 0.4 mM, the average molecular weight was 1.1 x 10(6) Da. However, an even greater increase of the PHB average molecular weight to 2.5 x 10(7) Da was observed without IPTG being added.-
dc.languageEnglish-
dc.publisherKluwer Academic Publishers-
dc.titleIncreased poly-beta-hydroxybutyrate (PHB) chain length by the modulation of PHA synthase activity in recombinant Escherichia coli-
dc.typeArticle-
dc.identifier.doi10.1023/A:1013752000022-
dc.description.journalClass1-
dc.identifier.bibliographicCitationBiotechnology Letters, v.23, no.24, pp.2057 - 2061-
dc.citation.titleBiotechnology Letters-
dc.citation.volume23-
dc.citation.number24-
dc.citation.startPage2057-
dc.citation.endPage2061-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscopus-
dc.identifier.wosid000173206600014-
dc.identifier.scopusid2-s2.0-0035693242-
dc.relation.journalWebOfScienceCategoryBiotechnology & Applied Microbiology-
dc.relation.journalResearchAreaBiotechnology & Applied Microbiology-
dc.type.docTypeArticle-
dc.subject.keywordPlusPOLYHYDROXYALKANOATES-
dc.subject.keywordPlusPOLYHYDROXYBUTYRATE-
dc.subject.keywordPlusBACTERIAL-
dc.subject.keywordPlusPLANTS-
dc.subject.keywordAuthoraverage molecular weight-
dc.subject.keywordAuthorPHA synthase-
dc.subject.keywordAuthorpolyhydroxybutyrate-
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KIST Article > 2001
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