Development of a local antibiotic delivery system using fibrin glue

Abstract

Fibrin glue (FG) has been successfully used in a wide range of surgical fields such as skin graft fixation, nerve repair, cartilage reattachment and microvascular anastomoses. The sustained release of antibiotics from FG for a longer period is desirable to avoid bacterial infection after application. Ampicillin sodium (AMP-Na) was selected as a model antibiotic and incorporated into FG. The incorporation of AMP-Na was attempted in two different ways. One was incorporation of AMP-Na in a dispersed state with the modification of the preparation method of FG. The other was incorporation of bovine serum albumin microspheres (BSA-MS) containing AMP-Na. The in vitro release of AMP-Na from various FGs and BSA MS was examined, The modifications of FG were found not to be appropriate methods to achieve sustained release of AMP-Na. The incorporation of crosslinked BSA-MS containing AMP-Na into FG was found to reduce the initial rapid release of AMP-Na and retarded release of AMP-Na.