Development and application of a high-performance liquid chromatography diode-array detection (HPLC-DAD) method for the simultaneous quantification of phenolic compounds in the aerial part of Glehnia littoralis

Authors
Park, Yun JiChoi, Yeong BinOh, Sang-BinMoon, JinyoungTruong, To QuyenHuynh, Phuong KimKim, Sang Min
Issue Date
2024-03
Publisher
한국응용생명화학회
Citation
Applied Biological Chemistry, v.67, no.1
Abstract
Glehnia littoralis, a medicinal herb employed in traditional practices for alleviating fatigue, cough, and a dry throat, is recognized for its beneficial properties due to a diverse array of active compounds found in its extracts. For example, the G. littoralis roots (Radix Glehniae) mainly contain coumarins and phenolic acids, serving as the primary focus of this study. Despite the widespread use of the tools in various industries and the development of multiple analytical methods for their examination, the edible aerial parts have industrial potential, and there is currently no analytical method available to identify their key components. In this study, a high-performance liquid chromatography method combined with diode array detection (HPLC-DAD) was developed to simultaneously detect 16 phenolic compounds previously reported to be present in the edible aerial parts of G. littoralis. The proposed approach included using gradient elution to change the solvent system from water/acetonitrile to water/methanol. Furthermore, the method validation was conducted, assessing its linearity, limit of detection, limit of quantification, precision, accuracy, and recovery, all of which demonstrated satisfactory results. Subsequently, the developed method was applied to quantify the phenolic compounds in various G. littoralis samples obtained from different organs, solvent extraction processes, and processing methods. Moreover, the online HPLC-ABTS (2,2MODIFIER LETTER PRIME-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assay was used to evaluate the antioxidant capacities of individual constituents, identifying four important antioxidants and estimate the overall antioxidant capacity of the G. littoralis extract.
Keywords
CONSTITUENTS; RADIX; High-performance liquid chromatography; Diode-array detection; Phenolic compound; Glehnia littoralis; Antioxidant activity
ISSN
2468-0834
URI
https://pubs.kist.re.kr/handle/201004/149570
DOI
10.1186/s13765-024-00884-9
Appears in Collections:
KIST Article > 2024
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