Double crystallization-driven copper-2-methylimidazole nanoflowers: Stabilizing glucose oxidase and activating nanozyme functions for tandem catalysis

Authors
Kim, DainKim, Byoung ChanHwang, Ee Taek
Issue Date
2025-06
Publisher
Elsevier BV
Citation
International Journal of Biological Macromolecules, v.315
Abstract
Based on the structural characteristics of metal-organic framework (MOF) synthesis, we designed a doublecrystallized copper-2-methylimidazole nanoflower (D-Cu NF) platform in which glucose oxidase (GOx) was incorporated to form an enzyme-nanozyme hybrid for glucose sensing. The D-Cu@GOx NF system mimics a GOx-horseradish peroxidase-like multi-enzyme cascade, benefiting from synergistic oxidation capabilities. Double crystallization of Cu nanoflowers (Cu-NFs) was crucial for inducing nanozyme activity by creating a unique Fenton-like reaction site, enhancing both cascade activity and enzyme stability. The system was constructed using a self-assembly method, integrating Cu-NF synthesis with in situ GOx immobilization. The double crystallization of Cu-NFs expanded the surface area, forming D-Cu@GOx NFs, which significantly enhanced cascade activity and enzyme stability. The system demonstrated excellent glucose detection performance, maintaining 88 % of enzyme activity after 30 days at room temperature, with temperature resistance up to 60 degrees C and pH stability between 3 and 8. The enhanced oxidation from the Cu metal Fenton-like reaction site enabled sensitive glucose detection over a wide linear range (0-50 mu M), with a limit of detection of 1.25 mu M. The system also showed high reproducibility, with a relative standard deviation of <5 % across five replicate measurements. Furthermore, it successfully detected human blood glucose in real samples, with results comparable to standard clinical methods. This report presents Cu NF synthesis with an integrated GOx approach, demonstrating costeffectiveness through enhanced stability and sensitivity that reduces enzyme usage and enables rapid, accurate glucose biosensing. The D-Cu@GOx NFs, a hybrid enzyme-nanozyme complex, offer improved sensitivity and stability for glucose detection in serum. By enhancing enzyme stability, the system eliminates the need for dual enzymes, reducing costs and improving efficiency, while maintaining cost-effectiveness for industrial and diagnostic applications.
Keywords
PEROXIDASE-LIKE ACTIVITY; METAL-ORGANIC FRAMEWORK; COLORIMETRIC DETECTION; CASCADE REACTIONS; SENSITIVE DETECTION; ENZYME; H2O2; NANOPARTICLES; Glucose oxidase (GOx); Enzyme-nanozyme hybrid system; Artificial enzyme cascade; Enzyme immobilization; Colorimetric glucose sensing
ISSN
0141-8130
URI
https://pubs.kist.re.kr/handle/201004/152656
DOI
10.1016/j.ijbiomac.2025.144341
Appears in Collections:
KIST Article > Others
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE