Protective Effect of Artemisia Asiatica Extract Against Renal Ischemia-Reperfusion Injury in Mice

Authors
Jang, Hyuk JaiJeong, Eui KyunKim, Seong SuLee, Ji HwanOh, Mi YoungKang, Ki SungKwan, Hak CheolSong, Kyung IlEom, Dae WoonHan, Duck Jong
Issue Date
2015-04
Publisher
BASKENT UNIV
Citation
EXPERIMENTAL AND CLINICAL TRANSPLANTATION, v.13, pp.377 - 382
Abstract
Objectives: An extract of Artemisia asiatica was reported to possess antioxidative and cytoprotective actions in various experiments. lschemia-reperfusion injury remains a major problem in kidney transplant, and the inflammatory response to ischemia-reperfusion injury exacerbates the resultant renal injury. In the present study, we investigated whether an extract of Artemisia asiatica exhibits renoprotective effects against ischemia-reperfusion-induced acute kidney injury in mice. Materials and Methods: Renal ischemia-reperfusion injury was induced in male C57BL/6 mice by bilateral renal pedicle occlusion for 30 minutes followed by reperfusion for 48 hours. An extract of Artemisia asiatica (100 mg/kg oral) was administered 4 days before ischemia-reperfusion injury. Sham operation and phosphate-buffered saline were used as controls. Blood and renal tissues were evaluated at 48 hours after ischemia-reperfusion injury. Results: Treatment with an extract of Artemisia asiatica significantly decreased blood urea nitrogen, serum creatinine levels, and kidney tubular injury (P <= .05). Western blot showed that an extract of Artemisia asiatica significantly increased the level of heme oxygenase-1 and B-cell lymphoma 2 at 48 hours after ischemia-reperfusion injury and attenuated the level of inducible nitric oxide synthase. Conclusions: An extract of Artemisia asiatica improves acute renal ischemia-reperfusion injury by reducing inflammation and apoptosis. These findings suggest that an extract of Artemisia asiatica is a potential therapeutic agent against acute ischemia-induced renal damage.
Keywords
APOPTOSIS; FAILURE; INFLAMMATION; KIDNEY; RATS; INHIBITION; INDUCTION; NECROSIS; DA-9601; APOPTOSIS; FAILURE; INFLAMMATION; KIDNEY; RATS; INHIBITION; INDUCTION; NECROSIS; DA-9601; Heme oxygenase-1; Inducible nitric oxide synthase; Kidney transplant; Renal failure
ISSN
1304-0855
URI
https://pubs.kist.re.kr/handle/201004/125574
DOI
10.6002/ect.mesot2014.P227
Appears in Collections:
KIST Article > 2015
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