Protective Effect of Artemisia Asiatica Extract Against Renal Ischemia-Reperfusion Injury in Mice
- Authors
- Jang, Hyuk Jai; Jeong, Eui Kyun; Kim, Seong Su; Lee, Ji Hwan; Oh, Mi Young; Kang, Ki Sung; Kwan, Hak Cheol; Song, Kyung Il; Eom, Dae Woon; Han, Duck Jong
- Issue Date
- 2015-04
- Publisher
- BASKENT UNIV
- Citation
- EXPERIMENTAL AND CLINICAL TRANSPLANTATION, v.13, pp.377 - 382
- Abstract
- Objectives: An extract of Artemisia asiatica was reported to possess antioxidative and cytoprotective actions in various experiments. lschemia-reperfusion injury remains a major problem in kidney transplant, and the inflammatory response to ischemia-reperfusion injury exacerbates the resultant renal injury. In the present study, we investigated whether an extract of Artemisia asiatica exhibits renoprotective effects against ischemia-reperfusion-induced acute kidney injury in mice. Materials and Methods: Renal ischemia-reperfusion injury was induced in male C57BL/6 mice by bilateral renal pedicle occlusion for 30 minutes followed by reperfusion for 48 hours. An extract of Artemisia asiatica (100 mg/kg oral) was administered 4 days before ischemia-reperfusion injury. Sham operation and phosphate-buffered saline were used as controls. Blood and renal tissues were evaluated at 48 hours after ischemia-reperfusion injury. Results: Treatment with an extract of Artemisia asiatica significantly decreased blood urea nitrogen, serum creatinine levels, and kidney tubular injury (P <= .05). Western blot showed that an extract of Artemisia asiatica significantly increased the level of heme oxygenase-1 and B-cell lymphoma 2 at 48 hours after ischemia-reperfusion injury and attenuated the level of inducible nitric oxide synthase. Conclusions: An extract of Artemisia asiatica improves acute renal ischemia-reperfusion injury by reducing inflammation and apoptosis. These findings suggest that an extract of Artemisia asiatica is a potential therapeutic agent against acute ischemia-induced renal damage.
- Keywords
- APOPTOSIS; FAILURE; INFLAMMATION; KIDNEY; RATS; INHIBITION; INDUCTION; NECROSIS; DA-9601; APOPTOSIS; FAILURE; INFLAMMATION; KIDNEY; RATS; INHIBITION; INDUCTION; NECROSIS; DA-9601; Heme oxygenase-1; Inducible nitric oxide synthase; Kidney transplant; Renal failure
- ISSN
- 1304-0855
- URI
- https://pubs.kist.re.kr/handle/201004/125574
- DOI
- 10.6002/ect.mesot2014.P227
- Appears in Collections:
- KIST Article > 2015
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.