A VISUAL MEMBRANE IMMUNOASSAY FOR THE DETECTION OF METHAMPHETAMINE USING AN ENZYME-LABELED TRACER DERIVED FROM METHAMPHETAMINE AND AMPHETAMINE

Authors
CHOI, MJGOROVITS, BMCHOI, JSONG, EYNAM, KSPARK, J
Issue Date
1994-07
Publisher
PHARMACEUTICAL SOC JAPAN
Citation
BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.17, no.7, pp.875 - 880
Abstract
A visual membrane enzyme immunoassay is described for the measurement of methamphetamine in urine. To increase assay sensitivity, tracers with chemically similar structures were cross-checked with the antibodies to determine their influence on the antibody binding. Tracers of horseradish peroxidase-labeled methamphetamine (MA-HRP) and amphetamine (A-HRP) derivatives were prepared for this purpose. Significant differences in antibody specificity were found between the two tracers. Based on the results of this study, a pair of an antibody and a tracer was selected and a membrane enzyme immunoassay (EIA) was developed utilizing the competitive binding between methamphetamine and the drug-HRP tracer. UltraBind membrane (0.45 mum) was used as the solid matrix to which the antibody was attached. Using diaminobenzidine substrate with Co2+ ion, a stable grey color appeared on the surface of membrane for MA-negative urine samples. No color appeared for MA-positive urine with a cut-off level of 0.8 ppm.
Keywords
REAGENT POLARIZATION FLUOROIMMUNOASSAY; URINE; REAGENT POLARIZATION FLUOROIMMUNOASSAY; URINE; METHAMPHETAMINE; AMPHETAMINE; IMMUNOGEN; TRACER INFLUENCE; VISUAL MEMBRANE IMMUNOASSAY
ISSN
0918-6158
URI
https://pubs.kist.re.kr/handle/201004/145550
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KIST Article > Others
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