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SYNTHESIS, NMR AND STRUCTURE OF OLIGONUCLEOTIDE PHOSPHORODITHIOATES

Authors
PIOTTO, ME; GRANGER, JN; CHO, Y; FARSCHTSCHI, N; GORENSTEIN, DG
Issue Date
1991-04-08
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
TETRAHEDRON, v.47, no.14-15, pp.2449 - 2461
Abstract
Thiophosphoramidite as well as thiophosphoramidate chemistry has been used to prepare dithiophosphate analogues of oligonucleotides. The H-1 and P-31 resonances of the 3'-thymidine phosphorodithioate decamer d(CGCTpS-2TpS-2AAGCG) were assigned by two-dimensional NMR and the solution structure determined.
Keywords
PURE-ABSORPTION-PHASE; RELAXATION MATRIX APPROACH; OVERHAUSER EFFECT SPECTRA; DINUCLEOSIDE PHOSPHORODITHIOATES; DEOXYNUCLEOSIDE PHOSPHORAMIDITES; OLIGODEOXYRIBONUCLEOSIDE METHYLPHOSPHONATES; PROTON RESONANCES; P-31 SIGNALS; ASSIGNMENT; DEOXYOLIGONUCLEOTIDES; PURE-ABSORPTION-PHASE; RELAXATION MATRIX APPROACH; OVERHAUSER EFFECT SPECTRA; DINUCLEOSIDE PHOSPHORODITHIOATES; DEOXYNUCLEOSIDE PHOSPHORAMIDITES; OLIGODEOXYRIBONUCLEOSIDE METHYLPHOSPHONATES; PROTON RESONANCES; P-31 SIGNALS; ASSIGNMENT; DEOXYOLIGONUCLEOTIDES; oligonucleotide phosphorodithioates; ³¹p NMR spectroscopy
ISSN
0040-4020
URI
https://pubs.kist.re.kr/handle/201004/146810
DOI
10.1016/S0040-4020(01)81780-4
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