Optimization of Environmentally Friendly Solvent-based Liposome Manufacturing System to Increase Bioavailability with Functional Materials

Abstract

Liposomes are one of the most intensively studied type of lipid nanovesicles. Liposomes increase the bioavailability of insoluble or poorly soluble materials, hence offering a wide range of food applications. In spite of such advantage, the use of liposome has been constrained by the fact that a toxic organic solvent is required during the manufacturing process to disperse the emulsifier. To this end, an environmentally friendly solvent-based liposome manufacturing system was developed and optimized. It was selected that a non-toxic solvent capable of dispersing phospholipids. Additionally, it was determined that the significant variables affecting the processing efficiency of liposomes using the Plackett-Burman (PB) design and the Response Surface Method (RSM) to optimize the process for mass production. As a result, liposomes with size and dispersion stability of approximately 180 nm and ？ 58 mV were obtained. Additionally, cells were treated with liposomes containing functional materials that is unstable or insoluble in water. In results, bioavailability was more than doubled in cell experiments compared to a functional material treatment.